Chromosomal mapping of 5S rDNA in two species of the genus Acanthocephalus (Echinorhynchida).
Acanthocephala
Fluorescence in situ hybridization
Multigene family
Ribosomal genes
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
29 Oct 2024
29 Oct 2024
Historique:
received:
02
07
2024
accepted:
16
10
2024
medline:
29
10
2024
pubmed:
29
10
2024
entrez:
29
10
2024
Statut:
epublish
Résumé
Chromosomal mapping of 5S rDNA in two Acanthocephala species was performed for the first time using fluorescence in situ hybridization (FISH) with a 5S rDNA probe. The 5S rDNA PCR products from the genomes of both species were sequenced and aligned and an identical 141 bp long coding region was determined. The same patterns of 5S rDNA gene cluster distribution were observed, with FISH signal restricted to a single autosomal chromosome pair. A preference for distal positioning on the chromosomes (subtelomeric position) was observed in both species. In addition, two-color FISH was performed to examine the mutual positions of 5S and 18S rDNA on the chromosomes. Our knowledge of the organization of the Acanthocephala genome is extremely limited and its chromosomes are poorly studied. Any new information about the location of chromosomal markers as important features of the respective karyotype may be useful in solving evolutionary questions.
Identifiants
pubmed: 39467878
doi: 10.1007/s00436-024-08381-0
pii: 10.1007/s00436-024-08381-0
doi:
Substances chimiques
RNA, Ribosomal, 5S
0
DNA, Ribosomal
0
RNA, Ribosomal, 18S
0
DNA, Helminth
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
362Subventions
Organisme : Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
ID : 2/0093/23
Organisme : Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
ID : 2/0093/23
Informations de copyright
© 2024. The Author(s).
Références
Bombarová M, Marec F, Nguyen P, Špakulová M (2007) Divergent location of ribosomal genes in chromosomes of fish thorny-headed worms, Pomphorhynchus laevis and Pomphorhynchus tereticollis (Acanthocephala). Genetica 131:141–149. https://doi.org/10.1007/s10709-006-9124-3
doi: 10.1007/s10709-006-9124-3
pubmed: 17143651
Ciganda M, Williams N (2011) Eukaryotic 5S rRNA biogenesis. Wiley Interdiscip Rev RNA 2:523–33. https://doi.org/10.1002/wrna.74
doi: 10.1002/wrna.74
pubmed: 21957041
pmcid: 3278907
García-Souto D, Pasantes JJ (2015) Molecular cytogenetics in digenean parasites: linked and unlinked major and 5S rDNAs, B chromosomes and karyotype diversification. Cytogenet Genome Res 147(2–3):195–207. https://doi.org/10.1159/000442504
doi: 10.1159/000442504
pubmed: 26680763
García-Souto D, Peréz-García C, Pasantes JJ (2017) Are pericentric inversions reorganizing wedge shell genomes? Genes 8(12):370. https://doi.org/10.3390/genes8120370
doi: 10.3390/genes8120370
pubmed: 29215567
pmcid: 5748688
Hooper DM, Price TD (2017) Chromosomal inversion differences correlate with range overlap in passerine birds. Nat Ecol Evol 1(10):1526–1534. https://doi.org/10.1038/s41559-017-0284-6
doi: 10.1038/s41559-017-0284-6
pubmed: 29185507
Huang K, Riesenberg LH (2020) Frequency, origins, and evolutionary role of chromosomal inversions in plants. Front Plant Sci 18(11):296. https://doi.org/10.3389/fpls.2020.00296
doi: 10.3389/fpls.2020.00296
Lisitsyna O, Barčák D, Orosová M, Fan CF, Oros M (2023) Acanthocephalans of marine and freshwater fishes from Taiwan with description of a new species. Folia Parasit 70:021. https://doi.org/10.14411/fp.2023.021
doi: 10.14411/fp.2023.021
Marková A, Orosová M, Marec F, Barčák D, Oros M (2024) Karyological study of Acanthocephalus lucii (Echinorhynchida): the occurrence of B chromosomes in populations from PCB-polluted waters. Diversity 16:140. https://doi.org/10.3390/d16030140
doi: 10.3390/d16030140
Nei M, Rooney AP (2005) Concerted and birth-and-death evolution of multigene families. Annu Rev Genet 39:121–152. https://doi.org/10.1146/annurev.genet.39.073003.112240
doi: 10.1146/annurev.genet.39.073003.112240
pubmed: 16285855
pmcid: 1464479
Orosová M, Špakulová M (2018) Tapeworm chromosomes: their value in systematics with instructions for cytogenetic study. Folia Parasit 65:001. https://doi.org/10.14411/fp.2018.001
doi: 10.14411/fp.2018.001
Orosová M, Marková A, Zrzavá M, Marec F, Oros M (2023) Chromosome analysis and the occurrence of B chromosomes in fish parasite Acanthocephalus anguillae (Palaeacanthocephala: Echinorhynchida). Parasite 30:44. https://doi.org/10.1051/parasite/2023045
doi: 10.1051/parasite/2023045
pubmed: 37870409
pmcid: 10592040
Perrot-Minnot MJ, Cozzarolo CS, Amin O et al (2023) Hooking the scientific community on thorny-headed worms: interesting and exciting facts, knowledge gaps and perspectives for research directions on Acanthocephala. Parasite 30:23. https://doi.org/10.1051/parasite/2023026
doi: 10.1051/parasite/2023026
pubmed: 37350678
pmcid: 10288976
Salvadori S, Deidda F, Carugati L, Melis R, Costa E, Sibiriu M, Coluccia E (2023) Chromosomal mapping of ribosomal clusters and telomeric sequences (TTAGG)
doi: 10.1080/24750263.2023.2217188
Tigano C, Rocco L, Ferrito V, Costagliola D, Pappalardo AM, Stingo V (2004) Chromosomal mapping and molecular characterization of ribosomal RNA genes in Lebias fasciata (Teleostei, Cyprinodontidae). Genetica 121:95–100. https://doi.org/10.1023/B:GENE.0000019931.89458.dc
doi: 10.1023/B:GENE.0000019931.89458.dc
pubmed: 15098742