A history-dependent integrase recorder of plant gene expression with single-cell resolution.
Arabidopsis
/ genetics
Gene Expression Regulation, Plant
Integrases
/ metabolism
Plant Stomata
/ genetics
Plant Roots
/ genetics
Single-Cell Analysis
/ methods
Plants, Genetically Modified
Arabidopsis Proteins
/ genetics
Promoter Regions, Genetic
/ genetics
Cell Differentiation
/ genetics
Genes, Reporter
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
30 Oct 2024
30 Oct 2024
Historique:
received:
05
06
2024
accepted:
18
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
epublish
Résumé
During development, most cells experience a progressive restriction of fate that ultimately results in a fully differentiated mature state. Understanding more about the gene expression patterns that underlie developmental programs can inform engineering efforts for new or optimized forms. Here, we present a four-state integrase-based recorder of gene expression history and demonstrate its use in tracking gene expression events in Arabidopsis thaliana in two developmental contexts: lateral root initiation and stomatal differentiation. The recorder uses two serine integrases to mediate sequential DNA recombination events, resulting in step-wise, history-dependent switching between expression of fluorescent reporters. By using promoters that express at different times along each of the two differentiation pathways to drive integrase expression, we tie fluorescent status to an ordered progression of gene expression along the developmental trajectory. In one snapshot of a mature tissue, our recorder is able to reveal past gene expression with single cell resolution. In this way, we are able to capture heterogeneity in stomatal development, confirming the existence of two alternate paths of differentiation.
Identifiants
pubmed: 39472426
doi: 10.1038/s41467-024-53716-1
pii: 10.1038/s41467-024-53716-1
doi:
Substances chimiques
Integrases
EC 2.7.7.-
Arabidopsis Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9362Informations de copyright
© 2024. The Author(s).
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