Johan Rockberg

Affiliations

KTH Royal Institute of Technology, Department of Protein Science, 10691 Stockholm, Sweden.

Publications (22)

Systematic use of synthetic 5'-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories.

Nucleic acids research

Avec: Peter Eisenhut , Aman Mebrahtu , Mona Moradi Barzadd , Niklas Thalén , Gerald Klanert , Marcus Weinguny , Anna Sandegren , Chao Su , Diane Hatton , Nicole Borth , Johan Rockberg

Enhanced metabolism and negative regulation of ER stress support higher erythropoietin production in HEK293 cells.

Cell reports

Avec: Rasool Saghaleyni , Magdalena Malm , Noah Moruzzi , Jan Zrimec , Ronia Razavi , Num Wistbacka , Hannes Thorell , Anton Pintar , Andreas Hober , Fredrik Edfors , Veronique Chotteau , Per-Olof Berggren , Luigi Grassi , Aleksej Zelezniak , Thomas Svensson , Diane Hatton , Jens Nielsen , Jonathan L Robinson , Johan Rockberg

An easy-to-use high-throughput selection system for the discovery of recombinant protein binders from alternative scaffold libraries.

Protein engineering, design & selection : PEDS

Avec: Marit Möller , Malin Jönsson , Magnus Lundqvist , Blenda Hedin , Louise Larsson , Emma Larsson , Johan Rockberg , Mathias Uhlén , Sarah Lindbo , Hanna Tegel , Sophia Hober

High throughput generation of a resource of the human secretome in mammalian cells.

New biotechnology

Avec: Hanna Tegel , Melanie Dannemeyer , Sara Kanje , Åsa Sivertsson , Anna Berling , Anne-Sophie Svensson , Andreas Hober , Henric Enstedt , Anna-Luisa Volk , Magnus Lundqvist , Mona Moradi , Delaram Afshari , Siri Ekblad , LanLan Xu , Malin Westin , Faranak Bidad , Lovisa Holmberg Schiavone , Rick Davies , Lorenz M Mayr , Sinead Knight , Sven O Göpel , Björn G Voldborg , Fredrik Edfors , Björn Forsström , Kalle von Feilitzen , Martin Zwahlen , Johan Rockberg , Jenny Ottosson Takanen , Mathias Uhlén , Sophia Hober

Ancestral lysosomal enzymes with increased activity harbor therapeutic potential for treatment of Hunter syndrome.

iScience

Avec: Natalie M Hendrikse , Anna Sandegren , Tommy Andersson , Jenny Blomqvist , Åsa Makower , Dominik Possner , Chao Su , Niklas Thalén , Agneta Tjernberg , Ulrica Westermark , Johan Rockberg , Stefan Svensson Gelius , Per-Olof Syrén , Erik Nordling

CaRA - A multi-purpose phage display library for selection of calcium-regulated affinity proteins.

New biotechnology

Avec: Malin Jönsson , Julia Scheffel , Emma Larsson , Marit Möller , Gabriella Rossi , Magnus Lundqvist , Johan Rockberg , Mathias Uhlén , Hanna Tegel , Sara Kanje , Sophia Hober

Mammalian cell display with automated oligo design and library assembly allows for rapid residue level conformational epitope mapping.

Communications biology

Avec: Niklas Berndt Thalén , Maximilian Karlander , Magnus Lundqvist , Helena Persson , Camilla Hofström , S Pauliina Turunen , Magdalena Godzwon , Anna-Luisa Volk , Magdalena Malm , Mats Ohlin , Johan Rockberg

The human secretome.

Science signaling

Avec: Mathias Uhlén , Max J Karlsson , Andreas Hober , Anne-Sophie Svensson , Julia Scheffel , David Kotol , Wen Zhong , Abdellah Tebani , Linnéa Strandberg , Fredrik Edfors , Evelina Sjöstedt , Jan Mulder , Adil Mardinoglu , Anna Berling , Siri Ekblad , Melanie Dannemeyer , Sara Kanje , Johan Rockberg , Magnus Lundqvist , Magdalena Malm , Anna-Luisa Volk , Peter Nilsson , Anna Månberg , Tea Dodig-Crnkovic , Elisa Pin , Martin Zwahlen , Per Oksvold , Kalle von Feilitzen , Ragna S Häussler , Mun-Gwan Hong , Cecilia Lindskog , Fredrik Ponten , Borbala Katona , Jimmy Vuu , Emil Lindström , Jens Nielsen , Jonathan Robinson , Burcu Ayoglu , Diana Mahdessian , Devin Sullivan , Peter Thul , Frida Danielsson , Charlotte Stadler , Emma Lundberg , Göran Bergström , Anders Gummesson , Bjørn G Voldborg , Hanna Tegel , Sophia Hober , Björn Forsström , Jochen M Schwenk , Linn Fagerberg , Åsa Sivertsson

Small-scale bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin.

Journal of biotechnology

Avec: Hubert Schwarz , Ye Zhang , Caijuan Zhan , Magdalena Malm , Raymond Field , Richard Turner , Christopher Sellick , Paul Varley , Johan Rockberg , Véronique Chotteau

SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping.

Nucleic acids research

Avec: Francis Jingxin Hu , Magnus Lundqvist , Mathias Uhlén , Johan Rockberg

Tuning of CHO secretional machinery improve activity of secreted therapeutic sulfatase 150-fold.

Metabolic engineering

Avec: Niklas Berndt Thalén , Mona Moradi Barzadd , Magnus Lundqvist , Johanna Rodhe , Monica Andersson , Gholamreza Bidkhori , Dominik Possner , Chao Su , Joakim Nilsson , Peter Eisenhut , Magdalena Malm , Alice Karlsson , Jeanette Vestin , Johan Forsberg , Erik Nordling , Adil Mardinoglu , Anna-Luisa Volk , Anna Sandegren , Johan Rockberg

Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers.

New biotechnology

Avec: Aman Mebrahtu , Gustav Aniander , Alessandro Mega , Mona Moradi Barzadd , Niklas Berndt Thalén , Lindvi Gudmundsdotter , Eva Backström Rydin , Anna Sandegren , Fredrik Y Frejd , Johan Rockberg

Systematic Development of Sandwich Immunoassays for the Plasma Secretome.

Proteomics

Avec: Ragna S Häussler , Annika Bendes , MariaJesus Iglesias , Laura Sanchez-Rivera , Tea Dodig-Crnković , Sanna Byström , Claudia Fredolini , Elin Birgersson , Matilda Dale , Fredrik Edfors , Linn Fagerberg , Johan Rockberg , Hanna Tegel , Mathias Uhlén , Ulrika Qundos , Jochen M Schwenk

Low Shear Stress Increases Recombinant Protein Production and High Shear Stress Increases Apoptosis in Human Cells.

iScience

Avec: Caijuan Zhan , Gholamreza Bidkhori , Hubert Schwarz , Magdalena Malm , Aman Mebrahtu , Ray Field , Christopher Sellick , Diane Hatton , Paul Varley , Adil Mardinoglu , Johan Rockberg , Veronique Chotteau

Evolution from adherent to suspension: systems biology of HEK293 cell line development.

Scientific reports

Avec: Magdalena Malm , Rasool Saghaleyni , Magnus Lundqvist , Marco Giudici , Veronique Chotteau , Ray Field , Paul G Varley , Diane Hatton , Luigi Grassi , Thomas Svensson , Jens Nielsen , Johan Rockberg

Small Bispecific Affinity Proteins for Simultaneous Target Binding and Albumin-Associated Half-Life Extension.

Molecular pharmaceutics

Avec: Emma von Witting , Sarah Lindbo , Magnus Lundqvist , Marit Möller , Andreas Wisniewski , Sara Kanje , Johan Rockberg , Hanna Tegel , Mikael Åstrand , Mathias Uhlén , Sophia Hober

Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins.

Metabolic engineering

Avec: Magdalena Malm , Chih-Chung Kuo , Mona Moradi Barzadd , Aman Mebrahtu , Num Wistbacka , Ronia Razavi , Anna-Luisa Volk , Magnus Lundqvist , David Kotol , Hanna Tegel , Sophia Hober , Fredrik Edfors , Torbjörn Gräslund , Veronique Chotteau , Ray Field , Paul G Varley , Robert G Roth , Nathan E Lewis , Diane Hatton , Johan Rockberg

Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion.

Scientific reports

Avec: Magnus Lundqvist , Niklas Thalén , Anna-Luisa Volk , Henning Gram Hansen , Eric von Otter , Per-Åke Nygren , Mathias Uhlen , Johan Rockberg

Reactive oxygen species as an initiator of toxic innate immune responses in retort to SARS-CoV-2 in an ageing population, consider N-acetylcysteine as early therapeutic intervention.

Toxicology reports

Avec: Aikaterini Nasi , Stephanie McArdle , Gustav Gaudernack , Gabriel Westman , Cornelis Melief , Johan Rockberg , Ramon Arens , Demetrios Kouretas , Jan Sjölin , Sara Mangsbo

Bispecific Antibody Molecule Inhibits Tumor Cell Proliferation More Efficiently Than the Two-Molecule Combination.

Drugs in R&D

Avec: Anna-Luisa Volk , Aman Mebrahtu , Bong-Kook Ko , Magnus Lundqvist , Maximilian Karlander , Hyun-Jong Lee , Fredrik Y Frejd , Kyu-Tae Kim , Jong-Seo Lee , Johan Rockberg

Secretome screening reveals immunomodulating functions of IFNα-7, PAP and GDF-7 on regulatory T-cells.

Scientific reports

Avec: Mei Ding , Rajneesh Malhotra , Tomas Ottosson , Magnus Lundqvist , Aman Mebrahtu , Johan Brengdahl , Ulf Gehrmann , Elisabeth Bäck , Douglas Ross-Thriepland , Ida Isaksson , Björn Magnusson , Kris F Sachsenmeier , Hanna Tegel , Sophia Hober , Mathias Uhlén , Lorenz M Mayr , Rick Davies , Johan Rockberg , Lovisa Holmberg Schiavone

Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells.

New biotechnology

Avec: Mona Moradi Barzadd , Magnus Lundqvist , Claire Harris , Magdalena Malm , Anna-Luisa Volk , Niklas Thalén , Veronique Chotteau , Luigi Grassi , Andrew Smith , Marina Leal Abadi , Giulia Lambiase , Suzanne Gibson , Diane Hatton , Johan Rockberg

Réseau de co-auteurs

Magnus Lundqvist 14 collaborations

Magdalena Malm 9 collaborations

Mathias Uhlén 8 collaborations

Hanna Tegel 8 collaborations

Anna-Luisa Volk 8 collaborations

Sophia Hober 7 collaborations

Aman Mebrahtu 6 collaborations

Diane Hatton 6 collaborations

Mona Moradi Barzadd 5 collaborations

Fredrik Edfors 5 collaborations

Veronique Chotteau 5 collaborations

Niklas Thalén 4 collaborations

Anna Sandegren 4 collaborations

Sara Kanje 4 collaborations

Chao Su 3 collaborations

Andreas Hober 3 collaborations

Luigi Grassi 3 collaborations

Jens Nielsen 3 collaborations

Marit Möller 3 collaborations

Niklas Berndt Thalén 3 collaborations

Adil Mardinoglu 3 collaborations

Ray Field 3 collaborations

Peter Eisenhut 2 collaborations

Rasool Saghaleyni 2 collaborations

Ronia Razavi 2 collaborations

Num Wistbacka 2 collaborations

Thomas Svensson 2 collaborations

Malin Jönsson 2 collaborations

Emma Larsson 2 collaborations

Sarah Lindbo 2 collaborations

Melanie Dannemeyer 2 collaborations

Åsa Sivertsson 2 collaborations

Anna Berling 2 collaborations

Anne-Sophie Svensson 2 collaborations

Siri Ekblad 2 collaborations

Lovisa Holmberg Schiavone 2 collaborations

Rick Davies 2 collaborations

Lorenz M Mayr 2 collaborations

Björn Forsström 2 collaborations

Kalle von Feilitzen 2 collaborations

Martin Zwahlen 2 collaborations

Dominik Possner 2 collaborations

Erik Nordling 2 collaborations

Julia Scheffel 2 collaborations

Maximilian Karlander 2 collaborations

David Kotol 2 collaborations

Ragna S Häussler 2 collaborations

Jochen M Schwenk 2 collaborations

Linn Fagerberg 2 collaborations

Hubert Schwarz 2 collaborations

Caijuan Zhan 2 collaborations

Christopher Sellick 2 collaborations

Paul Varley 2 collaborations

Gholamreza Bidkhori 2 collaborations

Fredrik Y Frejd 2 collaborations

Paul G Varley 2 collaborations

Gerald Klanert 1 collaboration

Marcus Weinguny 1 collaboration

Nicole Borth 1 collaboration

Noah Moruzzi 1 collaboration

Jan Zrimec 1 collaboration

Hannes Thorell 1 collaboration

Anton Pintar 1 collaboration

Per-Olof Berggren 1 collaboration

Aleksej Zelezniak 1 collaboration

Jonathan L Robinson 1 collaboration

Blenda Hedin 1 collaboration

Louise Larsson 1 collaboration

Henric Enstedt 1 collaboration

Mona Moradi 1 collaboration

Delaram Afshari 1 collaboration

LanLan Xu 1 collaboration

Malin Westin 1 collaboration

Faranak Bidad 1 collaboration

Sinead Knight 1 collaboration

Sven O Göpel 1 collaboration

Björn G Voldborg 1 collaboration

Jenny Ottosson Takanen 1 collaboration

Natalie M Hendrikse 1 collaboration

Tommy Andersson 1 collaboration

Jenny Blomqvist 1 collaboration

Åsa Makower 1 collaboration

Agneta Tjernberg 1 collaboration

Ulrica Westermark 1 collaboration

Stefan Svensson Gelius 1 collaboration

Per-Olof Syrén 1 collaboration

Gabriella Rossi 1 collaboration

Helena Persson 1 collaboration

Camilla Hofström 1 collaboration

S Pauliina Turunen 1 collaboration

Magdalena Godzwon 1 collaboration

Mats Ohlin 1 collaboration

Max J Karlsson 1 collaboration

Wen Zhong 1 collaboration

Abdellah Tebani 1 collaboration

Linnéa Strandberg 1 collaboration

Evelina Sjöstedt 1 collaboration

Jan Mulder 1 collaboration

Peter Nilsson 1 collaboration

Anna Månberg 1 collaboration

Tea Dodig-Crnkovic 1 collaboration

Elisa Pin 1 collaboration

Per Oksvold 1 collaboration

Mun-Gwan Hong 1 collaboration

Cecilia Lindskog 1 collaboration

Fredrik Ponten 1 collaboration

Borbala Katona 1 collaboration

Jimmy Vuu 1 collaboration

Emil Lindström 1 collaboration

Jonathan Robinson 1 collaboration

Burcu Ayoglu 1 collaboration

Diana Mahdessian 1 collaboration

Devin Sullivan 1 collaboration

Peter Thul 1 collaboration

Frida Danielsson 1 collaboration

Charlotte Stadler 1 collaboration

Emma Lundberg 1 collaboration

Göran Bergström 1 collaboration

Anders Gummesson 1 collaboration

Bjørn G Voldborg 1 collaboration

Ye Zhang 1 collaboration

Raymond Field 1 collaboration

Richard Turner 1 collaboration

Véronique Chotteau 1 collaboration

Francis Jingxin Hu 1 collaboration

Johanna Rodhe 1 collaboration

Monica Andersson 1 collaboration

Joakim Nilsson 1 collaboration

Alice Karlsson 1 collaboration

Jeanette Vestin 1 collaboration

Johan Forsberg 1 collaboration

Gustav Aniander 1 collaboration

Alessandro Mega 1 collaboration

Lindvi Gudmundsdotter 1 collaboration

Eva Backström Rydin 1 collaboration

Annika Bendes 1 collaboration

MariaJesus Iglesias 1 collaboration

Laura Sanchez-Rivera 1 collaboration

Tea Dodig-Crnković 1 collaboration

Sanna Byström 1 collaboration

Claudia Fredolini 1 collaboration

Elin Birgersson 1 collaboration

Matilda Dale 1 collaboration

Ulrika Qundos 1 collaboration

Marco Giudici 1 collaboration

Emma von Witting 1 collaboration

Andreas Wisniewski 1 collaboration

Mikael Åstrand 1 collaboration

Chih-Chung Kuo 1 collaboration

Torbjörn Gräslund 1 collaboration

Robert G Roth 1 collaboration

Nathan E Lewis 1 collaboration

Henning Gram Hansen 1 collaboration

Eric von Otter 1 collaboration

Per-Åke Nygren 1 collaboration

Mathias Uhlen 1 collaboration

Aikaterini Nasi 1 collaboration

Stephanie McArdle 1 collaboration

Gustav Gaudernack 1 collaboration

Gabriel Westman 1 collaboration

Cornelis Melief 1 collaboration

Ramon Arens 1 collaboration

Demetrios Kouretas 1 collaboration

Jan Sjölin 1 collaboration

Sara Mangsbo 1 collaboration

Bong-Kook Ko 1 collaboration

Hyun-Jong Lee 1 collaboration

Kyu-Tae Kim 1 collaboration

Jong-Seo Lee 1 collaboration

Mei Ding 1 collaboration

Rajneesh Malhotra 1 collaboration

Tomas Ottosson 1 collaboration

Johan Brengdahl 1 collaboration

Ulf Gehrmann 1 collaboration

Elisabeth Bäck 1 collaboration

Douglas Ross-Thriepland 1 collaboration

Ida Isaksson 1 collaboration

Björn Magnusson 1 collaboration

Kris F Sachsenmeier 1 collaboration

Claire Harris 1 collaboration

Andrew Smith 1 collaboration

Marina Leal Abadi 1 collaboration

Giulia Lambiase 1 collaboration

Suzanne Gibson 1 collaboration