CpxR negatively regulates the production of xenocoumacin 1, a dihydroisocoumarin derivative produced by Xenorhabdus nematophila.
CpxR
X. nematophila
antimicrobial activity
biosynthesis regulation
xenocoumacin 1
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
02
04
2018
revised:
07
05
2018
accepted:
19
05
2018
pubmed:
12
6
2018
medline:
13
4
2019
entrez:
12
6
2018
Statut:
ppublish
Résumé
Xenocoumacin 1 (Xcn1), a major antimicrobial compound produced by Xenorhabdus nematophila, has great potential for use in agricultural productions. In this study, we evaluated the effects of CpxR, a global response regulator associated with the mutualism and pathogenesis of X. nematophila, on the antimicrobial activity and Xcn1 production. The mutation of cpxR could promote the production of Xcn1 significantly with its level in ΔcpxR mutant being 3.07 times higher than that in the wild type. Additionally, the expression levels of xcnA-L genes, which are responsible for the production of Xcn1, were increased in ΔcpxR mutant while the expression levels of xcnMN, which are required for the conversion of Xcn1 into Xcn2 was reduced. Noticeably, Xcn2 was also enhanced on account of the conversion of excessive Xcn1 in spite of low expression levels of xcnM and xcnN in ΔcpxR mutant. The transcriptional levels of ompR and lrp, encoding the global response regulators OmpR and Lrp which negatively and positively regulate the production of Xcn1 were concurrently decreased and increased, respectively. Correspondingly, ΔcpxR mutant also exhibited increased antimicrobial activities in vitro and in vivo. Together, these findings suggest that CpxR negatively regulates xcnA-L genes expression while positively regulating xcnMN expression in X. nematophila YL001, which led to a high yield of Xcn1 in ΔcpxR mutant.
Identifiants
pubmed: 29888873
doi: 10.1002/mbo3.674
pmc: PMC6391269
doi:
Substances chimiques
Anti-Infective Agents
0
Bacterial Proteins
0
Benzopyrans
0
xenocoumacin 1
105688-01-5
CpxR protein, Bacteria
153554-07-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e00674Informations de copyright
© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
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