DCL-suppressed Nicotiana benthamiana plants: valuable tools in research and biotechnology.
Nicotiana benthamiana
CMV
DCL
Dicer
GFP
RNAi
TRV
TuMV
miRNA
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
pubmed:
22
10
2018
medline:
28
4
2020
entrez:
22
10
2018
Statut:
ppublish
Résumé
RNA silencing is a universal mechanism involved in development, epigenetic modifications and responses to biotic and abiotic stresses. The major components of this mechanism are Dicer-like (DCL), Argonaute (AGO) and RNA-dependent RNA polymerase (RDR) proteins. Understanding the role of each component is of great scientific and agronomic importance. Plants, including Nicotiana benthamiana, an important plant model, usually possess four DCL proteins, each of which has a specific role, namely being responsible for the production of an exclusive small RNA population. Here, we used RNA interference (RNAi) technology to target DCL proteins and produced single and combinatorial mutants for DCL. We analysed the phenotype for each DCL knockdown plant, together with the small RNA profile, by next-generation sequencing (NGS). We also investigated transgene expression, as well as viral infections, and were able to show that DCL suppression results in distinct developmental defects, changes in small RNA populations, increases in transgene expression and, finally, higher susceptibility in certain RNA viruses. Therefore, these plants are excellent tools for the following: (i) to study the role of DCL enzymes; (ii) to overexpress proteins of interest; and (iii) to understand the complex relationship between the plant silencing mechanism and biotic or abiotic stresses.
Identifiants
pubmed: 30343523
doi: 10.1111/mpp.12761
pmc: PMC6637889
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
432-446Informations de copyright
© 2018 The Authors. Molecular Plant Pathology Published by BSPP and John Wiley & Sons Ltd.
Références
Adv Virus Res. 2012;84:439-504
pubmed: 22682176
J Med Genet. 2018 Mar;55(3):143-149
pubmed: 29301855
EMBO J. 2006 Jul 26;25(14):3347-56
pubmed: 16810317
Trends Plant Sci. 2017 Dec;22(12):1056-1068
pubmed: 29032035
J Exp Bot. 2014 Feb;65(2):725-39
pubmed: 24376253
Cell Res. 2016 Nov;26(11):1264
pubmed: 27811943
PLoS One. 2013;8(3):e59534
pubmed: 23555698
Plant Methods. 2007 Oct 12;3:12
pubmed: 17931426
Cancer Res. 2004 Aug 1;64(15):5245-50
pubmed: 15289330
Cell. 2005 Dec 29;123(7):1279-91
pubmed: 16377568
Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6792-7
pubmed: 15096616
New Phytol. 2010 Apr;186(1):207-15
pubmed: 20409179
Genome Biol. 2009;10(3):R25
pubmed: 19261174
Plant Mol Biol. 1992 Dec;20(6):1203-7
pubmed: 1463857
Mol Plant Microbe Interact. 2017 Jan;30(1):63-71
pubmed: 27958768
J Gen Virol. 2009 Dec;90(Pt 12):3010-3014
pubmed: 19710258
Curr Genomics. 2015 Feb;16(1):23-31
pubmed: 25937811
Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12984-9
pubmed: 16129836
Plant J. 2015 Mar;81(5):781-93
pubmed: 25619543
Plant Physiol. 2017 Jun;174(2):1067-1081
pubmed: 28455401
BMC Genomics. 2015 Dec 01;16:1025
pubmed: 26626050
J Cell Physiol. 2016 Feb;231(2):303-13
pubmed: 26248304
Plant Biotechnol J. 2018 Feb;16(2):572-580
pubmed: 28710796
Biochim Biophys Acta. 2012 Feb;1819(2):137-48
pubmed: 21605713
Mol Plant Microbe Interact. 2008 Feb;21(2):178-87
pubmed: 18184062
Mol Plant Microbe Interact. 2008 Aug;21(8):1015-26
pubmed: 18616398
Genetics. 2018 Jan;208(1):1-18
pubmed: 29301946
Cell. 2010 Jan 8;140(1):111-22
pubmed: 20085706
J Exp Bot. 2010 Mar;61(5):1277-91
pubmed: 20097843
Plant Signal Behav. 2013 Aug;8(8):
pubmed: 23673353
Plant Cell. 2007 Jun;19(6):2053-63
pubmed: 17586651
Mol Plant Microbe Interact. 2002 Aug;15(8):826-33
pubmed: 12182340
J Integr Plant Biol. 2012 Mar;54(3):189-206
pubmed: 22313877
Plant J. 2018 Jul;95(2):204-218
pubmed: 29682831
Plant Cell. 2012 Sep;24(9):3575-89
pubmed: 23001036
Sci Rep. 2017 Jun 26;7(1):4240
pubmed: 28652582
Nat Plants. 2015 Nov 02;1:15165
pubmed: 27251536
Biochim Biophys Acta. 2013 Dec;1829(12):1300-8
pubmed: 24185199
PLoS Genet. 2013 Oct;9(10):e1003867
pubmed: 24204292
Mol Plant. 2013 Jan;6(1):232-4
pubmed: 23100483
Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2122-7
pubmed: 9122158
Biotechnol Adv. 2010 Jul-Aug;28(4):427-35
pubmed: 20152894
PLoS One. 2012;7(9):e46451
pubmed: 23029521
Nucleic Acids Res. 2006;34(21):6233-46
pubmed: 17090584
Virus Res. 2003 Jul;94(1):33-43
pubmed: 12837555
PLoS Biol. 2007 Mar;5(3):e57
pubmed: 17298187
BMC Plant Biol. 2014 Mar 11;14:60
pubmed: 24618068
PLoS Pathog. 2016 Oct 6;12(10):e1005935
pubmed: 27711201
Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17466-73
pubmed: 20870966
Mol Cell. 2005 Aug 5;19(3):421-8
pubmed: 16061187
J Virol. 2008 Jun;82(11):5167-77
pubmed: 18353962
J Biosci Bioeng. 2017 Aug;124(2):215-220
pubmed: 28336416
J Gen Virol. 2007 Jan;88(Pt 1):298-315
pubmed: 17170463
Nucleic Acids Res. 2005 Nov 27;33(20):e179
pubmed: 16314309
Nat Rev Microbiol. 2013 Nov;11(11):745-60
pubmed: 24129510
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Development. 1996 Sep;122(9):2631-8
pubmed: 8787738
Nat Genet. 2006 Jun;38(6):721-5
pubmed: 16699516
Curr Biol. 2005 Aug 23;15(16):1494-500
pubmed: 16040244
Trends Plant Sci. 2016 Dec;21(12):1034-1044
pubmed: 27793495
Evol Bioinform Online. 2015 Jan 07;11(Suppl 1):1-11
pubmed: 25673968
Biotechnol Lett. 2004 Mar;26(6):509-15
pubmed: 15127793
Methods Mol Biol. 2011;744:97-108
pubmed: 21533688
PLoS One. 2008 Mar 12;3(3):e1755
pubmed: 18335032
J Cell Physiol. 2018 Jun;233(6):4578-4594
pubmed: 29194606
Plant Physiol. 2005 Sep;139(1):296-305
pubmed: 16126864
PLoS One. 2018 Jan 2;13(1):e0190353
pubmed: 29293625
PLoS Biol. 2004 May;2(5):E104
pubmed: 15024409
Elife. 2015 Oct 02;4:e09591
pubmed: 26430765
Genome Res. 2015 Feb;25(2):235-45
pubmed: 25414514
Genome Res. 2009 Jan;19(1):57-69
pubmed: 18997003
Mol Cell. 2010 May 14;38(3):465-75
pubmed: 20381393
J Exp Bot. 2014 Dec;65(22):6655-66
pubmed: 25378580
Hum Vaccin Immunother. 2017 Feb;13(2):306-313
pubmed: 27929750
PLoS Pathog. 2016 Oct 12;12(10):e1005936
pubmed: 27732664
Plant Mol Biol. 2005 Nov;59(4):647-61
pubmed: 16244913
Plant J. 2012 Feb;69(3):462-74
pubmed: 21973320
Virology. 2000 Feb 1;267(1):29-35
pubmed: 10648180
Genes Dev. 2006 Dec 15;20(24):3407-25
pubmed: 17182867
Mol Gen Genet. 1993 Dec;241(5-6):504-14
pubmed: 8264525
Nucleic Acids Res. 2008 Nov;36(20):6429-38
pubmed: 18842626
Science. 2015 Apr 3;348(6230):120-3
pubmed: 25838384
Mol Plant Microbe Interact. 2012 Dec;25(12):1523-30
pubmed: 22876960
J Biomed Biotechnol. 2009;2009:952304
pubmed: 19859540
Mol Plant Pathol. 2010 Jul;11(4):577-83
pubmed: 20618713
Science. 2006 Jul 7;313(5783):68-71
pubmed: 16741077
Mol Plant Microbe Interact. 2014 Sep;27(9):944-55
pubmed: 24804808
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13079-84
pubmed: 9789044
Virus Genes. 2010 Feb;40(1):119-29
pubmed: 19859797
Plant J. 2015 Jan;81(2):223-32
pubmed: 25376953
Biotechnol Bioeng. 1997 Dec 5;56(5):473-84
pubmed: 18642268
PLoS Pathog. 2008 Apr 04;4(4):e1000038
pubmed: 18389061
Plant Sci. 2014 May;221-222:81-9
pubmed: 24656338
Sci Rep. 2017 Dec 11;7(1):17358
pubmed: 29229947
Plant Physiol. 2017 Nov;175(3):1424-1437
pubmed: 28928141
Plant Biotechnol J. 2005 Mar;3(2):259-73
pubmed: 17173625