Production, purification and biochemical characterization of a novel detergent-stable serine alkaline protease from Bacillus safensis strain RH12.

Bacillus / enzymology Bacterial Proteins / antagonists & inhibitors Calcium / pharmacology Coloring Agents / metabolism Cotton Fiber Detergents / pharmacology Drug Interactions Endopeptidases / biosynthesis Enzyme Inhibitors / pharmacology Enzyme Stability / drug effects Hydrogen-Ion Concentration Kinetics Phylogeny Polymers / pharmacology
Bacillus safensis Detergent Offshore oil field Protease Wash performance

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
Jan 2019
Historique:
received: 17 09 2018
revised: 18 10 2018
accepted: 18 10 2018
pubmed: 24 10 2018
medline: 18 1 2019
entrez: 24 10 2018
Statut: ppublish

Résumé

A novel extracellular protease (SAPRH) was hyper-produced (9000 U/mL) from Bacillus safensis RH12, a newly isolated enzyme from a Tunisian offshore oil field. The enzyme was purified to homogeneity, using salt-precipitation, heat-treatment and FPLC anion-exchange chromatography. The purified enzyme was a monomer of molecular mass of ~28 kDa. The NH

Identifiants

DOI: 10.1016/j.ijbiomac.2018.10.139 PMID: 30352229
pubmed: 30352229
pii: S0141-8130(18)34941-9
doi: 10.1016/j.ijbiomac.2018.10.139
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Coloring Agents 0
Detergents 0
Enzyme Inhibitors 0
Polymers 0
polyol 0
Endopeptidases EC 3.4.-
alkaline protease EC 3.4.99.-
Calcium SY7Q814VUP

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1227-1239

Informations de copyright

Copyright © 2018. Published by Elsevier B.V.

Auteurs

Hatem Rekik (H)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia; STE JMAL (EJM)-Laundry Detergent Industry, Z.I. Avenue August 13, Z.I. Poudriere 1, P.O. Box 407, Boustene, Sfax 3000, Tunisia.

Nadia Zaraî Jaouadi (N)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.

Fares Gargouri (F)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.

Wacim Bejar (W)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.

Fakher Frikha (F)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.

Najah Jmal (N)

STE JMAL (EJM)-Laundry Detergent Industry, Z.I. Avenue August 13, Z.I. Poudriere 1, P.O. Box 407, Boustene, Sfax 3000, Tunisia.

Samir Bejar (S)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.

Bassem Jaouadi (B)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address: bassem.jaouadi@cbs.rnrt.tn.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Production, purification and biochemical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Production, purification and biochemical...
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