Phylogenetic diversity of rhizobia nodulating native Mimosa gymnas grown in a South Brazilian ecotone.
16S rRNA
Betaproteobacteria
Burkholderia
Fabaceae
Genetic diversity
Symbiosis
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
30
08
2018
accepted:
15
11
2018
pubmed:
30
11
2018
medline:
3
7
2019
entrez:
30
11
2018
Statut:
ppublish
Résumé
Floristic surveys performed in "Campos Gerais" (Paraná, Brazil), an ecotone of Mata Atlântica and Cerrado biomes, highlights the richness and relative abundance of the family Fabaceae and point out the diversity and endemism of Mimosa spp. Our study reports the genetic diversity of rhizobia isolated from root nodules of native/endemic Mimosa gymnas Barneby in three areas of Guartelá State Park, an important conservation unit of "Campos Gerais". Soils of the sample areas were characterized as sandy, acid, poor in nutrients and organic matter. The genetic variability among the isolates was revealed by BOX-PCR genomic fingerprinting. Phylogeny based on 16S rRNA gene grouped the strains in a large cluster including Paraburkholderia nodosa and P. bannensis, while recA-gyrB phylogeny separated the strains in two groups: one including P. nodosa and the other without any described Paraburkholderia species. MLSA confirmed the separate position of this second group of strains within the genus Paraburkholderia and the nucleotide identity of the five concatened housekeeping genes was 95.9% in relation to P. nodosa BR 3437
Identifiants
pubmed: 30488371
doi: 10.1007/s11033-018-4506-z
pii: 10.1007/s11033-018-4506-z
doi:
Substances chimiques
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
Soil
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
529-540Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 465133/2014-4
Organisme : Fundação Araucária
ID : UEPG 09/2016
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : N/A
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