Genome-wide analysis of the Tetrahymena thermophila glutathione S-transferase gene superfamily.

Gene Duplication Genome, Protozoan Glutathione Transferase / chemistry Multigene Family Protozoan Proteins / chemistry Tetrahymena thermophila / enzymology
GST activity Gene expansion Glutathione S-transferases Phylogeny Tetrahymena thermophila

Journal

Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135

Informations de publication

Date de publication:
07 2019
Historique:
received: 14 08 2018
revised: 21 11 2018
accepted: 30 11 2018
pubmed: 21 12 2018
medline: 24 12 2019
entrez: 21 12 2018
Statut: ppublish

Résumé

The ciliate Tetrahymena thermophila has a rapid response to detoxify xenobiotics, which presents opportunity to study the diversification of Glutathione S-Transferase superfamily. In-silico identification of putative GST genes were resulted with 70 GST genes; 49 TtGSTmu, 7 TtGSTomega, 5 TtGSTtheta, 2 TtGSTzeta, 4 TtMAPEG and 3 TtEF1G. TtGST superfamily has short intron carrying or intronless genes. The most expressed mRNAs of TtGST are limited to 4 members at all life stages. TtGST genes are widely distributed to all five micronuclear chromosomes with the highest diversified members from different classes in chromosome 4. The clustering and the orientation of some TtGSTs in the T. thermophila genome give clues about the recent gene duplication. Analysis of GSH affinity-purified GST proteins with Western blot and activity assay showed GST activity carrying purified TtGST populations. In conclusion, the enhanced genome capacity of TtGST superfamily may have evolved through improved GST enzymatic activity.

Identifiants

DOI: 10.1016/j.ygeno.2018.11.034 PMID: 30572113
pubmed: 30572113
pii: S0888-7543(18)30476-2
doi: 10.1016/j.ygeno.2018.11.034
pii:
doi:

Substances chimiques

Protozoan Proteins 0
Glutathione Transferase EC 2.5.1.18

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

534-548

Informations de copyright

Copyright © 2018 Elsevier Inc. All rights reserved.

Auteurs

Ayça Fulya Üstüntanır Dede (AF)

Department of Biology, Graduate School of Sciences, Eskisehir Technical University, Yunusemre Campus, Eskisehir 26470, Turkey.

Muhittin Arslanyolu (M)

Department of Biology, Faculty of Sciences, Eskisehir Technical University, Yunusemre Campus, Eskisehir 26470, Turkey. Electronic address: marslanyolu@anadolu.edu.tr.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Duplication de gène › Genome-wide analysis of the Tetrahymena...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome de protozoaire › Genome-wide analysis of the Tetrahymena...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Alkyl et aryl transferases › Glutathione transferase › Genome-wide analysis of the Tetrahymena...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Famille multigénique › Genome-wide analysis of the Tetrahymena...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de protozoaire › Genome-wide analysis of the Tetrahymena...
questionsmedicales.fr › Eucaryotes › Alveolata › Ciliophora › Oligohymenophorea › Hymenostomatida › Tetrahymenina › Tetrahymena › Tetrahymena thermophila › Genome-wide analysis of the Tetrahymena...