Insight into the effect of glycerol on stability of globular proteins in high protein model system.

Calorimetry, Differential Scanning Dietary Proteins / chemistry Glycerol / chemistry Hydrophobic and Hydrophilic Interactions Lactalbumin / chemistry Lactoferrin / chemistry Lactoglobulins / chemistry Muramidase / chemistry Protein Denaturation Protein Folding Protein Stability Protein Structure, Secondary Tryptophan
Glycerol High protein food Lactoferrin Lysozyme Thermal stability α-Lactalbumin β-Lactoglobulin

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
25 Apr 2019
Historique:
received: 17 09 2018
revised: 24 11 2018
accepted: 26 11 2018
entrez: 26 12 2018
pubmed: 26 12 2018
medline: 12 2 2019
Statut: ppublish

Résumé

A model system was established to investigate the effect of glycerol on stability of globular proteins. To study whether protective effect of glycerol is dependent on the nature of protein, four kinds of globular protein were used including α-lactalbumin, β-lactoglobulin, native lactoferrin and lysozyme. Differential scanning calorimetry (DSC) was used to study the thermal stability of the proteins with glycerol aqueous (0-80% v/v). The results showed that the stability of β-lactoglobulin and lysozyme (hydrophobicity-dominated proteins) increased with glycerol concentration. However, that of α-lactalbumin and lactoferrin (metalloproteins), regardless of the ion-binding state, initially increased and then decreased. Different protective effects were observed beyond 50% (v/v) glycerol concentrations in liquid phase. The secondary structure and tryptophan exposure remained unchanged. In conclusion, the protection of glycerol on protein stability was highly dependent on the nature of proteins.

Identifiants

DOI: 10.1016/j.foodchem.2018.11.117 PMID: 30583443
pubmed: 30583443
pii: S0308-8146(18)32062-4
doi: 10.1016/j.foodchem.2018.11.117
pii:
doi:

Substances chimiques

Dietary Proteins 0
Lactoglobulins 0
Tryptophan 8DUH1N11BX
Lactalbumin 9013-90-5
Muramidase EC 3.2.1.17
Lactoferrin EC 3.4.21.-
Glycerol PDC6A3C0OX

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

780-785

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Xiaoxia Chen (X)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China; International Joint Research Laboratory for Functional Dairy Protein Ingredients, U.S. China Dairy Innovation Center, Jiangnan University, Wuxi, Jiangsu Province 214122, China.

Bhesh Bhandari (B)

School of Agriculture and Food Science, University of Queensland, Brisbane, Queensland 4072, Australia.

Peng Zhou (P)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China; International Joint Research Laboratory for Functional Dairy Protein Ingredients, U.S. China Dairy Innovation Center, Jiangnan University, Wuxi, Jiangsu Province 214122, China. Electronic address: zhoupeng@jiangnan.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse thermique différentielle › Calorimétrie différentielle à balayage › Insight into the effect of glycerol on...
questionsmedicales.fr › Aliments et boissons › Aliments › Protéines alimentaires › Insight into the effect of glycerol on...
questionsmedicales.fr › Glucides › Polyols › Dérivés du glycérol › Glycérol › Insight into the effect of glycerol on...
questionsmedicales.fr › Phénomènes chimiques › Interactions hydrophobes et hydrophiles › Insight into the effect of glycerol on...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Lactalbumine › Insight into the effect of glycerol on...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Métalloprotéines › Protéines de liaison au fer › Transferrines › Lactoferrine › Insight into the effect of glycerol on...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Lactoglobulines › Insight into the effect of glycerol on...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Lysozyme › Insight into the effect of glycerol on...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › Dépliement des protéines › Dénaturation des protéines › Insight into the effect of glycerol on...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › Insight into the effect of glycerol on...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Insight into the effect of glycerol on...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › Insight into the effect of glycerol on...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Tryptophane › Insight into the effect of glycerol on...