Impact of Expanded Small Alkyl-Binding Pocket by Triple Point Mutations on Substrate Specificity of Thermoanaerobacter ethanolicus Secondary Alcohol Dehydrogenase.

Acetophenones / chemistry Alcohol Dehydrogenase / genetics Alcohol Oxidoreductases / genetics Alcohols / chemistry Bacterial Proteins / genetics Catalytic Domain / genetics Enzyme Assays Enzyme Stability Genetic Engineering Ketones / chemistry Kinetics Models, Molecular Molecular Conformation Mutagenesis, Site-Directed Point Mutation Sequence Analysis, DNA Substrate Specificity Thermoanaerobacter / enzymology
Computer-aided modeling alcohol dehydrogenase asymmetric reduction substrate specificity thermostability

Journal

Journal of microbiology and biotechnology
ISSN: 1738-8872
Titre abrégé: J Microbiol Biotechnol
Pays: Korea (South)
ID NLM: 9431852

Informations de publication

Date de publication:
28 Mar 2019
Historique:
pubmed: 6 1 2019
medline: 27 4 2019
entrez: 6 1 2019
Statut: ppublish

Résumé

Site-directed mutagenesis was employed to generate five different triple point mutations in the double mutant (C295A/I86A) of

Identifiants

DOI: 10.4014/jmb.1812.12016 PMID: 30609883
pubmed: 30609883
pii: 10.4014/jmb.1812.12016
doi: 10.4014/jmb.1812.12016
doi:

Substances chimiques

Acetophenones 0
Alcohols 0
Bacterial Proteins 0
Ketones 0
Alcohol Oxidoreductases EC 1.1.-
Alcohol Dehydrogenase EC 1.1.1.1
isopropanol dehydrogenase (NADP) EC 1.1.1.80
acetophenone RK493WHV10

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

373-381

Auteurs

Amos Dwamena (A)

Department of Chemical & Biological Engineering, Hanbat National University, Daejeon 34158, Republic of Korea.
Department of Chemistry and Biochemistry, South Dakota State University, SD 57007, USA.

Robert Phillips (R)

Department of Chemistry, University of Georgia, Athens, GA 30602, USA.
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.

Chang Sup Kim (CS)

Department of Chemical & Biological Engineering, Hanbat National University, Daejeon 34158, Republic of Korea.

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Classifications MeSH

questionsmedicales.fr › Composés chimiques organiques › Cétones › Acétophénones › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Alcohol oxidoreductases › NAD (+) and NADP (+) dependent Alcohol oxidoreductases › Alcohol dehydrogenase › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Alcohol oxidoreductases › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Dosages enzymatiques › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Composés chimiques organiques › Cétones › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Mutagenèse dirigée › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Mutation ponctuelle › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Impact of Expanded Small Alkyl-Binding Pocket...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes irréguliers à Gram positif › Thermoanaerobacter › Impact of Expanded Small Alkyl-Binding Pocket...