Genomic analysis of three Clostridioides difficile isolates from urban water sources.

Bacteriological Techniques Berlin Clostridioides difficile / classification Genome, Bacterial Genomics Genotype Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Water Microbiology
Clostridioides difficile Genomes Sediment Wastewater

Journal

Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216

Informations de publication

Date de publication:
Apr 2019
Historique:
received: 28 08 2018
revised: 05 11 2018
accepted: 04 01 2019
pubmed: 12 1 2019
medline: 25 7 2019
entrez: 12 1 2019
Statut: ppublish

Résumé

We investigated inflow of a wastewater treatment plant and sediment of an urban lake for the presence of Clostridioides difficile by cultivation and PCR. Among seven colonies we sequenced the complete genomes of three: two non-toxigenic isolates from wastewater and one toxigenic isolate from the urban lake. For all obtained isolates, a close genomic relationship with human-derived isolates was observed.

Identifiants

DOI: 10.1016/j.anaerobe.2019.01.002 PMID: 30633971
pubmed: 30633971
pii: S1075-9964(19)30002-2
doi: 10.1016/j.anaerobe.2019.01.002
pii:
doi:

Types de publication

Journal Article

Langues

eng

Pagination

22-26

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Daniela Numberger (D)

Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315, Berlin, Germany.

Thomas Riedel (T)

Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.

Gayle McEwen (G)

Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315, Berlin, Germany.

Ulrich Nübel (U)

Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.

Martinique Frentrup (M)

Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.

Isabel Schober (I)

Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.

Boyke Bunk (B)

Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.

Cathrin Spröer (C)

Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.

Jörg Overmann (J)

Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.

Hans-Peter Grossart (HP)

Leibniz Institute of Freshwater Ecology and Inland Fisheries, Zur alten Fischerhütte 2, 16775, Stechlin, Germany; University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Strasse 24-25, 14476, Potsdam, Germany. Electronic address: hgrossart@igb-berlin.de.

Alex D Greenwood (AD)

Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315, Berlin, Germany; Freie Universität Berlin, Department of Veterinary Medicine, Institute for Virology, Robert-von-Ostertag-Strasse 7-13, 14163, Berlin, Germany. Electronic address: greenwood@izw-berlin.de.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques microbiologiques › Techniques bactériologiques › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Régions géographiques › Europe › Allemagne › Berlin › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Bactéries › Firmicutes › Clostridiales › Clostridioides › Clostridium difficile › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Disciplines des sciences naturelles › Disciplines des sciences biologiques › Biologie › Génétique › Génomique › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Phénomènes génétiques › Génotype › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Genomic analysis of three Clostridioides...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Microbiologie de l'eau › Genomic analysis of three Clostridioides...