Transposon insertional mutagenesis in
Journal
Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
11
11
2017
accepted:
03
01
2019
pubmed:
13
1
2019
medline:
14
6
2019
entrez:
13
1
2019
Statut:
ppublish
Résumé
To understand how complex genetic networks perform and regulate diverse cellular processes, the function of each individual component must be defined. Comprehensive phenotypic studies of mutant alleles have been successful in model organisms in determining what processes depend on the normal function of a gene. These results are often ported to newly sequenced genomes by using sequence homology. However, sequence similarity does not always mean identical function or phenotype, suggesting that new methods are required to functionally annotate newly sequenced species. We have implemented comparative analysis by high-throughput experimental testing of gene dispensability in
Identifiants
pubmed: 30635343
pii: gr.232330.117
doi: 10.1101/gr.232330.117
pmc: PMC6396416
doi:
Substances chimiques
DNA Transposable Elements
0
Fungal Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
396-406Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM120430
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : CIHR
Pays : Canada
Informations de copyright
© 2019 Sanchez et al.; Published by Cold Spring Harbor Laboratory Press.
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