High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium.
Bacterial communities
Ciliates
Human pathogens and commensals
Metabarcoding
Microbiomes
Single cell sequencing
Journal
Microbial ecology
ISSN: 1432-184X
Titre abrégé: Microb Ecol
Pays: United States
ID NLM: 7500663
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
05
10
2018
accepted:
07
01
2019
pubmed:
21
1
2019
medline:
19
7
2019
entrez:
21
1
2019
Statut:
ppublish
Résumé
Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of several strains of Paramecium (Ciliophora) originating from different locations and belonging to two morpho-species by high-throughput sequencing (HTS) of the 16S rRNA gene. Possible reasons of HTS results bias were addressed comparing DNA libraries obtained using different primers and different number of ciliate cells. Microbiomes associated with ciliates and their environments were always significantly different by prokaryotic taxonomic composition and bacterial richness. There were also pronounced differences between Paramecium strains. Interestingly, potentially pathogenic bacteria were revealed in Paramecium microbiomes.
Identifiants
pubmed: 30661111
doi: 10.1007/s00248-019-01321-x
pii: 10.1007/s00248-019-01321-x
doi:
Substances chimiques
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
286-298Subventions
Organisme : Russian Science Foundation
ID : 16-14-10157
Organisme : Russian Foundation for Basic Research
ID : 14-04-01796
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