Oxygen and contact with human intestinal epithelium independently stimulate virulence gene expression in enteroaggregative Escherichia coli.
Adhesins, Escherichia coli
/ genetics
Bacterial Toxins
/ metabolism
Cell Line, Tumor
Colon
/ microbiology
Enterotoxins
/ metabolism
Escherichia coli
/ genetics
Escherichia coli Infections
/ microbiology
Escherichia coli Proteins
/ genetics
Fimbriae, Bacterial
/ genetics
Gene Expression Regulation, Bacterial
Hemolysin Proteins
/ metabolism
Host-Pathogen Interactions
Humans
Intestinal Mucosa
/ microbiology
Intestine, Small
/ microbiology
Oxygen
/ metabolism
Polysaccharide-Lyases
/ metabolism
Serine Endopeptidases
/ metabolism
Trans-Activators
/ genetics
Virulence Factors
/ genetics
EAEC
adherence
colonic epithelium
diarrhoea
gene expression
oxygen
virulence
Journal
Cellular microbiology
ISSN: 1462-5822
Titre abrégé: Cell Microbiol
Pays: India
ID NLM: 100883691
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
15
06
2018
revised:
14
12
2018
accepted:
14
01
2019
pubmed:
24
1
2019
medline:
12
8
2020
entrez:
24
1
2019
Statut:
ppublish
Résumé
Enteroaggregative Escherichia coli (EAEC) are important intestinal pathogens causing acute and persistent diarrhoeal illness worldwide. Although many putative EAEC virulence factors have been identified, their association with pathogenesis remains unclear. As environmental cues can modulate bacterial virulence, we investigated the effect of oxygen and human intestinal epithelium on EAEC virulence gene expression to determine the involvement of respective gene products in intestinal colonisation and pathogenesis. Using in vitro organ culture of human intestinal biopsies, we established the colonic epithelium as the major colonisation site of EAEC strains 042 and 17-2. We subsequently optimised a vertical diffusion chamber system with polarised T84 colon carcinoma cells for EAEC infection and showed that oxygen induced expression of the global regulator AggR, aggregative adherence fimbriae, E. coli common pilus, EAST-1 toxin, and dispersin in EAEC strain 042 but not in 17-2. Furthermore, the presence of T84 epithelia stimulated additional expression of the mucinase Pic and the toxins HlyE and Pet. This induction was dependent on physical host cell contact and did not require AggR. Overall, these findings suggest that EAEC virulence in the human gut is modulated by environmental signals including oxygen and the intestinal epithelium.
Identifiants
pubmed: 30673154
doi: 10.1111/cmi.13012
pmc: PMC6563437
doi:
Substances chimiques
AAP protein, E coli
0
Adhesins, Escherichia coli
0
AggR protein, E coli
0
Bacterial Toxins
0
Enterotoxins
0
Escherichia coli Proteins
0
Hemolysin Proteins
0
Trans-Activators
0
Virulence Factors
0
heat stable toxin (E coli)
0
hlyE protein, E coli
0
Pet protein, E coli
EC 3.4.21.-
Serine Endopeptidases
EC 3.4.21.-
Polysaccharide-Lyases
EC 4.2.2.-
hyaluronate lyase
EC 4.2.2.1
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13012Subventions
Organisme : Medical Research Council
ID : MR/J002062/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M018261/1
Pays : United Kingdom
Informations de copyright
© 2019 The Authors Cellular Microbiology Published by John Wiley & Sons Ltd.
Références
Protein Expr Purif. 2004 Feb;33(2):223-31
pubmed: 14711510
J Infect Dis. 1995 Feb;171(2):465-8
pubmed: 7844392
Int J Med Microbiol. 2010 Nov;300(7):440-8
pubmed: 20452276
Curr Opin Microbiol. 2018 Feb;41:83-88
pubmed: 29258058
Int J Environ Res Public Health. 2015 Jul 23;12(8):8631-43
pubmed: 26213951
Mol Microbiol. 2003 May;48(4):947-58
pubmed: 12753188
J Infect Dis. 1988 Jul;158(1):70-9
pubmed: 2899125
Infect Immun. 2010 Oct;78(10):4101-9
pubmed: 20696826
Infect Immun. 1991 Dec;59(12):4310-7
pubmed: 1937792
Infect Immun. 1996 Nov;64(11):4761-8
pubmed: 8890237
Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10637-42
pubmed: 17563352
Mol Microbiol. 2007 Dec;66(5):1123-35
pubmed: 17986189
Infect Immun. 2008 Oct;76(10):4378-84
pubmed: 18591223
Microbiology (Reading). 2009 Sep;155(Pt 9):2895-2906
pubmed: 19542001
J Infect Dis. 1985 Sep;152(3):560-5
pubmed: 2863319
Commun Dis Public Health. 1999 Jun;2(2):108-13
pubmed: 10402744
Infect Immun. 1999 Nov;67(11):5587-96
pubmed: 10531204
Cell Microbiol. 2019 Jun;21(6):e13012
pubmed: 30673154
mBio. 2017 Jun 6;8(3):
pubmed: 28588132
Virulence. 2013 May 15;4(4):315-23
pubmed: 23552827
Cell Microbiol. 2014 Aug;16(8):1255-66
pubmed: 24612002
Infect Immun. 1992 May;60(5):2083-91
pubmed: 1348724
Environ Microbiol. 2010 Sep;12(9):2426-35
pubmed: 20406285
Infect Immun. 2010 Nov;78(11):4958-64
pubmed: 20823198
J Clin Microbiol. 2003 May;41(5):2138-40
pubmed: 12734261
Am J Trop Med Hyg. 2009 Feb;80(2):294-301
pubmed: 19190229
J Bacteriol. 1990 Nov;172(11):6333-8
pubmed: 2172211
Infect Immun. 2015 Apr;83(4):1396-405
pubmed: 25624357
Infect Immun. 2015 Mar;83(3):942-9
pubmed: 25534942
Infect Immun. 2013 Jan;81(1):122-32
pubmed: 23090962
Lett Appl Microbiol. 2007 Oct;45(4):405-10
pubmed: 17897383
Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4586-91
pubmed: 10200306
Gut Microbes. 2011 Jan-Feb;2(1):13-24
pubmed: 21637014
Infect Immun. 1996 Nov;64(11):4751-60
pubmed: 8890236
Eur J Clin Microbiol Infect Dis. 2007 Dec;26(12):901-6
pubmed: 17899229
FEMS Immunol Med Microbiol. 2012 Dec;66(3):281-98
pubmed: 22775224
Biochem Biophys Res Commun. 2005 Mar 25;328(4):1154-62
pubmed: 15707998
Nat Rev Microbiol. 2011 Apr;9(4):265-78
pubmed: 21407243
Mol Cell Pediatr. 2016 Dec;3(1):14
pubmed: 27002817
Res Microbiol. 1994 Jun-Aug;145(5-6):437-50
pubmed: 7855430
Mol Microbiol. 2011 Jul;81(1):179-91
pubmed: 21542864
Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):5503-8
pubmed: 25870295
Mol Microbiol. 2006 Sep;61(5):1267-82
pubmed: 16925558
Proc Natl Acad Sci U S A. 1990 Jun;87(11):4304-8
pubmed: 2349239
Lancet Infect Dis. 2011 Sep;11(9):671-6
pubmed: 21703928
Infect Immun. 2014 Apr;82(4):1719-24
pubmed: 24516112
J Bacteriol. 2007 Mar;189(6):2262-73
pubmed: 17220229
J Bacteriol. 1994 Aug;176(15):4691-9
pubmed: 7913930
Nature. 2010 May 20;465(7296):355-8
pubmed: 20436458
Infect Immun. 2007 Oct;75(10):4891-9
pubmed: 17698572
Clin Microbiol Rev. 2014 Jul;27(3):614-30
pubmed: 24982324
Gut Microbes. 2013 Jul-Aug;4(4):281-91
pubmed: 23680797
BMC Microbiol. 2009 Dec 09;9:252
pubmed: 20003185
J Clin Invest. 2002 Nov;110(9):1329-37
pubmed: 12417572
Mol Microbiol. 2019 Feb;111(2):534-551
pubmed: 30485564
Pediatr Infect Dis J. 1987 Sep;6(9):829-31
pubmed: 3313248
Cell Microbiol. 2011 Feb;13(2):171-6
pubmed: 21166974
Nat Rev Microbiol. 2013 Mar;11(3):205-12
pubmed: 23411864
J Infect Dis. 1998 Jan;177(1):88-96
pubmed: 9419174
Gastroenterology. 1987 May;92(5 Pt 1):1133-45
pubmed: 3557010