A well supported multi gene phylogeny of 52 dictyostelia.

Ancestral state reconstruction Dictyostelia Dictyostelium caveatum Phylogenetic marker genes Phylogenomics Polysphondylium multicystogenum

Journal

Molecular phylogenetics and evolution
ISSN: 1095-9513
Titre abrégé: Mol Phylogenet Evol
Pays: United States
ID NLM: 9304400

Informations de publication

Date de publication:
05 2019
Historique:
received: 11 10 2018
revised: 09 01 2019
accepted: 21 01 2019
pubmed: 4 2 2019
medline: 14 6 2019
entrez: 4 2 2019
Statut: ppublish

Résumé

The Dictyostelid social amoebas are a popular model system for cell- and developmental biology and for evolution of sociality. Small subunit (SSU) ribosomal DNA-based phylogenies subdivide the known 150 species into four major and some minor groups, but lack resolution within groups, particularly group 4, and, as shown by genome-based phylogenies of 11 species, showed errors in the position of the root and nodes separating major clades. We are interested in the evolution of cell-type specialization, which particularly expanded in group 4. To construct a more robust phylogeny, we first included 7 recently sequenced genomes in the genome-based phylogeny of 47 functionally divergent proteins and next selected 6 proteins (Agl, AmdA, PurD, PurL, RpaA, SmdA) that independently or in sets of two fully reproduced the core-phylogeny. We amplified their coding regions from 34 Dictyostelium species and combined their concatenated sequences with those identified in the 18 genomes to generate a fully resolved phylogeny. The new AAPPRS based phylogeny (after the acronym of the 6 proteins) subdivides group 4 into 2 branches. These branches further resolve into 5 clades, rather than the progressively nested group 4 topology of the SSU rDNA tree, and also re-orders taxa in the other major groups. Ancestral state reconstruction of 25 phenotypic traits returned higher "goodness of fit" metrics for evolution of 19 of those traits over the AAPPRS tree, than over the SSU rDNA tree. The novel tree provides a solid framework for studying the evolution of cell-type specialization, signalling and other cellular processes in particularly group 4, which contains the model Dictyostelid D. discoideum.

Identifiants

pubmed: 30711536
pii: S1055-7903(18)30654-7
doi: 10.1016/j.ympev.2019.01.017
pmc: PMC6430600
pii:
doi:

Substances chimiques

Protozoan Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

66-73

Subventions

Organisme : Wellcome Trust
ID : 100293/Z/12/Z
Pays : United Kingdom

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

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Auteurs

Christina Schilde (C)

School of Life Sciences, University of Dundee, Dundee DD15EH, UK.

Hajara M Lawal (HM)

School of Life Sciences, University of Dundee, Dundee DD15EH, UK.

Koryu Kin (K)

School of Life Sciences, University of Dundee, Dundee DD15EH, UK.

Ikumi Shibano-Hayakawa (I)

Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan; Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.

Kei Inouye (K)

Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.

Pauline Schaap (P)

School of Life Sciences, University of Dundee, Dundee DD15EH, UK. Electronic address: p.schaap@dundee.ac.uk.

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Classifications MeSH