LiSEQ - whole-genome sequencing of a cross-sectional survey of Listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe.


Journal

Microbial genomics
ISSN: 2057-5858
Titre abrégé: Microb Genom
Pays: England
ID NLM: 101671820

Informations de publication

Date de publication:
02 2019
Historique:
pubmed: 19 2 2019
medline: 14 9 2019
entrez: 19 2 2019
Statut: ppublish

Résumé

We present the LiSEQ (Listeria SEQuencing) project, funded by the European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in the European Union from ready-to-eat foods, compartments along the food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report the molecular characterization of a selection of this data set employing whole-genome sequencing analysis. We present an overview of the strain diversity observed in different sampled sources, and characterize the isolates based on their virulence and resistance profile. We integrate into our analysis the global L. monocytogenes genome collection described by Moura and colleagues in 2016 to assess the representativeness of the LiSEQ collection in the context of known L. monocytogenes strain diversity.

Identifiants

pubmed: 30775964
doi: 10.1099/mgen.0.000257
pmc: PMC6421348
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Anaïs Painset (A)

1​Public Health England Gastrointestinal Bacteria Reference Unit, 61 Colindale Avenue, London, NW9 5EQ, UK.
2​National Institute for Health Research Health Protection Research Unit (NIHR HPRU) in Gastrointestinal Infections at University of Liverpool, Liverpool, UK.

Jonas T Björkman (JT)

3​Statens Serum Institut, 5 Artillerivej, DK-2300, Copenhagen S, Denmark.

Kristoffer Kiil (K)

3​Statens Serum Institut, 5 Artillerivej, DK-2300, Copenhagen S, Denmark.

Laurent Guillier (L)

4​Maisons-Alfort Laboratory for Food Safety, Salmonella and Listeria Unit, University of Paris-Est, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Maisons-Alfort, France.

Jean-François Mariet (JF)

4​Maisons-Alfort Laboratory for Food Safety, Salmonella and Listeria Unit, University of Paris-Est, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Maisons-Alfort, France.

Benjamin Félix (B)

4​Maisons-Alfort Laboratory for Food Safety, Salmonella and Listeria Unit, University of Paris-Est, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Maisons-Alfort, France.

Corinne Amar (C)

1​Public Health England Gastrointestinal Bacteria Reference Unit, 61 Colindale Avenue, London, NW9 5EQ, UK.

Ovidiu Rotariu (O)

5​School of Biological Sciences, The University of Aberdeen, Cruickshank Building. St Machar Drive, Aberdeen, Scotland, AB24 3UU, UK.

Sophie Roussel (S)

4​Maisons-Alfort Laboratory for Food Safety, Salmonella and Listeria Unit, University of Paris-Est, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Maisons-Alfort, France.

Francisco Perez-Reche (F)

6​Institute of Complex Systems and Mathematical Biology, SUPA, School of Natural and Computing Sciences, University of Aberdeen, Aberdeen, Scotland, UK.

Sylvain Brisse (S)

7​Institut Pasteur, Paris, France.

Alexandra Moura (A)

7​Institut Pasteur, Paris, France.

Marc Lecuit (M)

7​Institut Pasteur, Paris, France.

Ken Forbes (K)

8​School of Medicine and Dentistry, The University of Aberdeen, Foresterhill, Aberdeen, Scotland, AB25 2ZD, UK.

Norval Strachan (N)

5​School of Biological Sciences, The University of Aberdeen, Cruickshank Building. St Machar Drive, Aberdeen, Scotland, AB24 3UU, UK.

Kathie Grant (K)

1​Public Health England Gastrointestinal Bacteria Reference Unit, 61 Colindale Avenue, London, NW9 5EQ, UK.
2​National Institute for Health Research Health Protection Research Unit (NIHR HPRU) in Gastrointestinal Infections at University of Liverpool, Liverpool, UK.

Eva Møller-Nielsen (E)

3​Statens Serum Institut, 5 Artillerivej, DK-2300, Copenhagen S, Denmark.

Timothy J Dallman (TJ)

2​National Institute for Health Research Health Protection Research Unit (NIHR HPRU) in Gastrointestinal Infections at University of Liverpool, Liverpool, UK.
1​Public Health England Gastrointestinal Bacteria Reference Unit, 61 Colindale Avenue, London, NW9 5EQ, UK.

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