Assembly and functionality of the ribosome with tethered subunits.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 02 2019
25 02 2019
Historique:
received:
15
08
2018
accepted:
25
01
2019
entrez:
27
2
2019
pubmed:
26
2
2019
medline:
18
4
2019
Statut:
epublish
Résumé
Ribo-T is an engineered ribosome whose small and large subunits are tethered together by linking 16S rRNA and 23S rRNA in a single molecule. Although Ribo-T can support cell proliferation in the absence of wild type ribosomes, Ribo-T cells grow slower than those with wild type ribosomes. Here, we show that cell growth defect is likely explained primarily by slow Ribo-T assembly rather than its imperfect functionality. Ribo-T maturation is stalled at a late assembly stage. Several post-transcriptional rRNA modifications and some ribosomal proteins are underrepresented in the accumulated assembly intermediates and rRNA ends are incompletely trimmed. Ribosome profiling of Ribo-T cells shows no defects in translation elongation but reveals somewhat higher occupancy by Ribo-T of the start codons and to a lesser extent stop codons, suggesting that subunit tethering mildly affects the initiation and termination stages of translation. Understanding limitations of Ribo-T system offers ways for its future development.
Identifiants
pubmed: 30804338
doi: 10.1038/s41467-019-08892-w
pii: 10.1038/s41467-019-08892-w
pmc: PMC6389949
doi:
Substances chimiques
Codon, Initiator
0
RNA, Bacterial
0
RNA, Ribosomal, 16S
0
RNA, Ribosomal, 23S
0
Ribosomal Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
930Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM127134
Pays : United States
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