Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity.
Journal
Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838
Informations de publication
Date de publication:
19 Mar 2019
19 Mar 2019
Historique:
pubmed:
8
3
2019
medline:
29
3
2019
entrez:
8
3
2019
Statut:
ppublish
Résumé
We demonstrated that 19 out of 20 RNA residues in the guide region of crRNA can be replaced with DNA residues with high GC-contents. The cellular activity of the chimeric crRNAs to disrupt the target gene was comparable to that of the native crRNA.
Substances chimiques
RNA, Guide
0
DNA
9007-49-2
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM