Validation and Stabilization of a Prophage Lysin of Clostridium perfringens by Using Yeast Surface Display and Coevolutionary Models.
Clostridium perfringens
antimicrobial protein
coevolutionary model
homolog
lysin
Journal
Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801
Informations de publication
Date de publication:
15 05 2019
15 05 2019
Historique:
received:
08
01
2019
accepted:
27
02
2019
pubmed:
10
3
2019
medline:
10
5
2020
entrez:
10
3
2019
Statut:
epublish
Résumé
Bacteriophage lysins are compelling antimicrobial proteins whose biotechnological utility and evolvability would be aided by elevated stability. Lysin catalytic domains, which evolved as modular entities distinct from cell wall binding domains, can be classified into one of several families with highly conserved structure and function, many of which contain thousands of annotated homologous sequences. Motivated by the quality of these evolutionary data, the performance of generative protein models incorporating coevolutionary information was analyzed to predict the stability of variants in a collection of 9,749 multimutants across 10 libraries diversified at different regions of a putative lysin from a prophage region of a
Identifiants
pubmed: 30850429
pii: AEM.00054-19
doi: 10.1128/AEM.00054-19
pmc: PMC6498168
pii:
doi:
Substances chimiques
Viral Proteins
0
Endopeptidases
EC 3.4.-
endolysin
EC 3.4.99.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : P30 CA077598
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM121777
Pays : United States
Informations de copyright
Copyright © 2019 American Society for Microbiology.
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