Papain-like cysteine proteases prepare plant cyclic peptide precursors for cyclization.
RiPP biosynthesis
cyclotide
kalata B1
papain-like cysteine protease
plant defensins
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
16 04 2019
16 04 2019
Historique:
pubmed:
5
4
2019
medline:
9
4
2020
entrez:
5
4
2019
Statut:
ppublish
Résumé
Cyclotides are plant defense peptides that have been extensively investigated for pharmaceutical and agricultural applications, but key details of their posttranslational biosynthesis have remained elusive. Asparaginyl endopeptidases are crucial in the final stage of the head-to-tail cyclization reaction, but the enzyme(s) involved in the prerequisite steps of N-terminal proteolytic release were unknown until now. Here we use activity-guided fractionation to identify specific members of papain-like cysteine proteases involved in the N-terminal cleavage of cyclotide precursors. Through both characterization of recombinantly produced enzymes and
Identifiants
pubmed: 30944220
pii: 1901807116
doi: 10.1073/pnas.1901807116
pmc: PMC6475389
doi:
Substances chimiques
Cyclotides
0
Defensins
0
Plant Proteins
0
Cysteine Proteases
EC 3.4.-
Papain
EC 3.4.22.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7831-7836Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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