CRISPR/Cas9-mediated functional recovery of the recessive rc allele to develop red rice.
rc
CRISPR/Cas9
functional recovery
proanthocyanidins
red rice
Journal
Plant biotechnology journal
ISSN: 1467-7652
Titre abrégé: Plant Biotechnol J
Pays: England
ID NLM: 101201889
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
15
12
2018
revised:
24
03
2019
accepted:
29
03
2019
pubmed:
20
4
2019
medline:
28
2
2020
entrez:
20
4
2019
Statut:
ppublish
Résumé
Red rice contains high levels of proanthocyanidins and anthocyanins, which have been recognized as health-promoting nutrients. The red coloration of rice grains is controlled by two complementary genes, Rc and Rd. The RcRd genotype produces red pericarp in wild species Oryza rufipogon, whereas most cultivated rice varieties produce white grains resulted from a 14-bp frame-shift deletion in the seventh exon of the Rc gene. In the present study, we developed a CRISPR/Cas9-mediated method to functionally restore the recessive rc allele through reverting the 14-bp frame-shift deletion to in-frame mutations in which the deletions were in multiples of three bases, and successfully converted three elite white pericarp rice varieties into red ones. Rice seeds from T
Identifiants
pubmed: 31002444
doi: 10.1111/pbi.13125
pmc: PMC6790373
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2096-2105Informations de copyright
© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
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