Dynamic changes of metabolomics and expression of candicidin biosynthesis gene cluster caused by the presence of a pleiotropic regulator AdpA in Streptomyces ZYJ-6.

Bacterial Proteins / genetics Candicidin / biosynthesis Gene Expression Regulation, Bacterial Metabolomics Multigene Family Streptomyces / genetics Trans-Activators / genetics
Candicidin FBA Metabolomics Streptomyces qRT-PCR

Journal

Bioprocess and biosystems engineering
ISSN: 1615-7605
Titre abrégé: Bioprocess Biosyst Eng
Pays: Germany
ID NLM: 101088505

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 29 12 2018
accepted: 15 04 2019
pubmed: 8 5 2019
medline: 26 12 2019
entrez: 8 5 2019
Statut: ppublish

Résumé

Candicidin is one of the frequent antibiotics for its high antifungal activity, but the productivity is still extremely low. Introduction of adpA into Streptomyces ZYJ-6 could improve candicidin productivity significantly and achieved 9338 μg/mL, which was the highest value ever reported in the literature. Combined analyses of transcriptional levels, metabolic flux and metabolomics indicate that para-aminobenzoic acid and the first step of shikimic acid metabolism were not the bottleneck for the candicidin production in the control. However, methylmalonyl-CoA played a central role in the candicidin production and the gene methB responsible for the biosynthesis of methylmalonyl-CoA might be the candidate gene target for further improving the production of candicidin.

Identifiants

DOI: 10.1007/s00449-019-02135-4 PMID: 31062087
pubmed: 31062087
doi: 10.1007/s00449-019-02135-4
pii: 10.1007/s00449-019-02135-4
doi:

Substances chimiques

Bacterial Proteins 0
Trans-Activators 0
Candicidin 1403-17-4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1353-1365

Subventions

Organisme : NWO-MoST Joint Program
ID : 2013DFG32630
Organisme : the National Basic Research Program of China (973 Program)
ID : No. 2012CB721000G
Organisme : National Key Special Program
ID : 2017YFF 0204600

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Auteurs

Xiaoyun Liu (X)

State Key Laboratory of Bioreactor Engineering, School of Biochemical Engineering, East China University of Science and Technology, Room 413, Building 18, Shanghai, 200237, People's Republic of China.

Xiaojuan Sun (X)

State Key Laboratory of Bioreactor Engineering, School of Biochemical Engineering, East China University of Science and Technology, Room 413, Building 18, Shanghai, 200237, People's Republic of China.

Weimin He (W)

State Key Laboratory of Bioreactor Engineering, School of Biochemical Engineering, East China University of Science and Technology, Room 413, Building 18, Shanghai, 200237, People's Republic of China.

Xiwei Tian (X)

State Key Laboratory of Bioreactor Engineering, School of Biochemical Engineering, East China University of Science and Technology, Room 413, Building 18, Shanghai, 200237, People's Republic of China.

Yingping Zhuang (Y)

State Key Laboratory of Bioreactor Engineering, School of Biochemical Engineering, East China University of Science and Technology, Room 413, Building 18, Shanghai, 200237, People's Republic of China.

Ju Chu (J)

State Key Laboratory of Bioreactor Engineering, School of Biochemical Engineering, East China University of Science and Technology, Room 413, Building 18, Shanghai, 200237, People's Republic of China. juchu@ecust.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Dynamic changes of metabolomics and expression...
questionsmedicales.fr › Composés chimiques organiques › Lactones › Candicidine › Dynamic changes of metabolomics and expression...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Dynamic changes of metabolomics and expression...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Métabolomique › Dynamic changes of metabolomics and expression...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Famille multigénique › Dynamic changes of metabolomics and expression...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Streptomycetaceae › Streptomyces › Dynamic changes of metabolomics and expression...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales régulatrices ou accessoires › Transactivateurs › Dynamic changes of metabolomics and expression...