Effects of the microbicide ceragenin CSA-13 on and properties of Bacillus subtilis spores prepared on two very different media.
Bacillus
antimicrobials
bacterial spores
disinfection
spores
sporulation media
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
19
07
2018
revised:
23
03
2019
accepted:
30
04
2019
pubmed:
9
5
2019
medline:
20
8
2019
entrez:
9
5
2019
Statut:
ppublish
Résumé
To determine how the microbicide ceragenin-13 (CSA-13) kills Bacillus subtilis spores prepared on growth or sporulation media, and these spores' properties. Spores made on Luria broth (LB) growth or double-strength Schaeffer's-glucose (2xSG) sporulation plates found that spores made on LB plates have coat defects as evidenced by their lower hypochlorite resistance, faster germination with dodecylamine and slower germination with Ca CSA-13 kills bacterial spores, but only at high concentrations and temperatures, and is preceded by CaDPA release. CSA-13 is not a direct sporicide as reported previously, but most likely germinates spores via activation of spores' CaDPA channel, albeit inefficiently, and then killing the germinated spores.
Substances chimiques
Amines
0
Anti-Infective Agents
0
Culture Media
0
Picolinic Acids
0
Steroids
0
ceragenin CSA-13
0
dipicolinic acid
UE81S5CQ0G
dodecylamine
YWY9OD6A2K
Types de publication
Journal Article
Langues
eng
Pagination
109-120Subventions
Organisme : Program of Qingjiang excellent young talents
Organisme : Jiangxi University of Science and Technology
Organisme : China Scholarship Council
ID : 201708360022
Organisme : National Natural Science Foundation of China
ID : 31500421
Informations de copyright
© 2019 The Society for Applied Microbiology.