Engineering the morphology and metabolism of pH tolerant Ustilago cynodontis for efficient itaconic acid production.
Itaconate
Metabolic engineering
Morphology
Ustilago cynodontis
Ustilago maydis
pH-tolerance
Journal
Metabolic engineering
ISSN: 1096-7184
Titre abrégé: Metab Eng
Pays: Belgium
ID NLM: 9815657
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
25
02
2019
revised:
01
04
2019
accepted:
06
05
2019
pubmed:
16
5
2019
medline:
9
4
2020
entrez:
16
5
2019
Statut:
ppublish
Résumé
Besides Aspergillus terreus and Ustilago maydis, Ustilago cynodontis is also known as a natural itaconate producer. U. cynodontis was reported as one of the best itaconate producing species in the family of the Ustilaginaceae, featuring a relatively high pH tolerance in comparison to other smut fungi. However, in contrast to U. maydis, it readily displays filamentous growth under sub-optimal growth conditions. In this study, U. cynodontis is established as efficient pH-tolerant itaconic acid producer through a combination of morphological and metabolic engineering. Deletions of the genes ras2, fuz7, and ubc3 abolished the filamentous growth of U. cynodontis, leading to a stable yeast-like growth under a range of stress-inducing conditions. The yeast-like morphology was also maintained in a pulsed fed batch production of 21 g L
Identifiants
pubmed: 31091468
pii: S1096-7176(19)30084-9
doi: 10.1016/j.ymben.2019.05.004
pii:
doi:
Substances chimiques
Fungal Proteins
0
Succinates
0
itaconic acid
Q4516562YH
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
293-300Informations de copyright
Copyright © 2019 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.