Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides.
Journal
Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838
Informations de publication
Date de publication:
11 Jun 2019
11 Jun 2019
Historique:
pubmed:
28
5
2019
medline:
31
7
2019
entrez:
25
5
2019
Statut:
ppublish
Résumé
PAGE and UV melting analysis revealed that longer LNA-based splice-switching oligonucleotides (SSOs) formed secondary structures by themselves, reducing their effective concentration. To avoid such secondary structure formation, we introduced 7-deaza-2'-deoxyguanosine or 2'-deoxyinosine into the SSOs. These modified SSOs, with fewer secondary structures, showed higher exon skipping activities.
Substances chimiques
Oligonucleotides
0
locked nucleic acid
0
Inosine
5A614L51CT
7-deaza-2'-deoxyguanosine
7668FG8W4U
Deoxyguanosine
G9481N71RO
deoxyinosine
HN0RQ6SBWQ
Types de publication
Journal Article
Langues
eng