Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes).

Ambisense Bipartite genome Endogenous virus element Evolution High-throughput sequencing Lentinula edodes Mymonaviridae Negative-strand RNA virus Phenuiviridae Shitake mushroom

Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
07 2019
Historique:
received: 14 03 2019
revised: 21 04 2019
accepted: 19 05 2019
pubmed: 4 6 2019
medline: 5 11 2019
entrez: 2 6 2019
Statut: ppublish

Résumé

There is still limited information on the diversity of (-)ssRNA viruses that infect fungi. Here, we have discovered two novel (-)ssRNA mycoviruses in the shiitake mushroom (Lentinula edodes). The first virus has a monopartite RNA genome and relates to that of mymonaviruses (Mononegavirales), especially to Hubei rhabdo-like virus 4 from arthropods and thus designated as Lentinula edodes negative-strand RNA virus 1. The second virus has a putative bipartite RNA genome and is related to the recently discovered bipartite or tripartite phenui-like viruses (Bunyavirales) associated with plants and ticks, and designated as Lentinula edodes negative-strand RNA virus 2 (LeNSRV2). LeNSRV2 is likely the first segmented (-)ssRNA virus known to infect fungi. Its smaller RNA segment encodes a putative nucleocapsid and a plant MP-like protein using a potential ambisense coding strategy. These findings enhance our understanding of the diversity, evolution and spread of (-)ssRNA viruses in fungi.

Identifiants

pubmed: 31153047
pii: S0042-6822(19)30128-X
doi: 10.1016/j.virol.2019.05.008
pii:
doi:

Substances chimiques

RNA, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

125-136

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Yu-Hsin Lin (YH)

Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.

Miki Fujita (M)

Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.

Sotaro Chiba (S)

Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan; Asian Satellite Campuses Institute, Nagoya University, Nagoya 464-8601, Japan.

Kiwamu Hyodo (K)

Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.

Ida Bagus Andika (IB)

Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.

Nobuhiro Suzuki (N)

Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.

Hideki Kondo (H)

Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan. Electronic address: hkondo@okayama-u.ac.jp.

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Classifications MeSH