Characterisation of ParB encoded on multipartite genome in Deinococcus radiodurans and their roles in radioresistance.
Amino Acid Sequence
Bacterial Proteins
/ genetics
Chromosomes, Bacterial
/ genetics
Cloning, Molecular
Deinococcus
/ genetics
Escherichia coli
/ genetics
Gene Expression Regulation, Bacterial
Genes, Bacterial
/ genetics
Plasmids
Protein Binding
Protein Interaction Domains and Motifs
Protein Structure, Secondary
Sequence Alignment
Sequence Deletion
Centromere binding protein
Deinococcus
Genome segregation
Interdependence of genome maintenance and cell division
Multipartite genome system
Radioresistance
Toroidal nucleoid
Journal
Microbiological research
ISSN: 1618-0623
Titre abrégé: Microbiol Res
Pays: Germany
ID NLM: 9437794
Informations de publication
Date de publication:
Historique:
received:
14
10
2018
revised:
27
02
2019
accepted:
16
03
2019
entrez:
11
6
2019
pubmed:
11
6
2019
medline:
30
7
2019
Statut:
ppublish
Résumé
The Deinococcus radiodurans multipartite genome consists of 2 chromosomes and 2 plasmids Its genome encodes 4 ParA and 4 ParB proteins on different replicons. Multiple sequence alignments of ParBs encoded on these genome elements showed that ParB of primary chromosome (ParB1) is close to chromosomal type ParB and is found to be different from ParBs encoded on chromosome II (ParB2) and megaplasmid (ParB3) elements. We observed that ParB1, ParB2 and ParB3 exist as dimer in solution and these proteins interact to self but not to its homologs in D. radiodurans, suggesting the specificity in ParBs dimerization. The parB1 deletion mutant showed slow growth under normal condition and relatively reduced resistance to γ-radiation as compared to wild type. The parB2 and parB3 mutants maintained without selection pressure showed loss of radioresistance, which was not observed when maintained with selection pressure. Nearly half of the populations of these mutants showed resistance to antibiotics marked to respective genome elements. Interestingly, all the parB mutants showed increased copy numbers of cognate genome element in cells maintained with antibiotics possibly due to arrest in genome segregation. These results suggested that ParB proteins encoded on multipartite genome system in D. radiodurans form homodimer and not heterodimer with other ParB homologs, and they independently regulate the segregation of respective genome elements. The roles of ParB1 proteins in normal as well as radiation stressed growth of this bacterium have also been ascertained.
Identifiants
pubmed: 31178048
pii: S0944-5013(18)31209-6
doi: 10.1016/j.micres.2019.03.005
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Pagination
22-32Informations de copyright
Copyright © 2019 Elsevier GmbH. All rights reserved.