A Framework for the Modular and Combinatorial Assembly of Synthetic Gene Circuits.
DNA assembly
Gibson assembly
cloning
gene circuit
synthetic biology
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
19 07 2019
19 07 2019
Historique:
pubmed:
12
6
2019
medline:
12
6
2020
entrez:
12
6
2019
Statut:
ppublish
Résumé
Synthetic gene circuits emerge from iterative design-build-test cycles. Most commonly, the time-limiting step is the circuit construction process. Here, we present a hierarchical cloning scheme based on the widespread Gibson assembly method and make the set of constructed plasmids freely available. Our two-step modular cloning scheme allows for simple, fast, efficient, and accurate assembly of gene circuits and combinatorial circuit libraries in
Identifiants
pubmed: 31185158
doi: 10.1021/acssynbio.9b00174
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM