Identification of Burkholderia thailandensis with novel genotypes in the soil of central Sierra Leone.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
06
02
2019
accepted:
18
04
2019
revised:
11
07
2019
pubmed:
15
6
2019
medline:
28
11
2019
entrez:
15
6
2019
Statut:
epublish
Résumé
The soil-dwelling bacillus Burkholderia pseudomallei is the etiological-agent of the neglected and life-threatening emerging infection melioidosis. The distribution of B. pseudomallei in West Africa is unknown. In the present study we aimed to determine whether B. pseudomallei and B. thailandensis are present in the environment of central Sierra Leone. In June-July 2017, we conducted an environmental surveillance study-designed in accordance with existing consensus guidelines-in central Sierra Leone. A total of 1,000 soil samples (100 per site) were collected and cultured. B. pseudomallei was not identified in the soil, but we identified seven novel B. thailandensis sequence types with multi-locus sequence typing (MLST) and 16S rRNA gene sequence analyses. The presence of B. pseudomallei was not demonstrated, however, multiple novel B. thailandensis sequence types were identified. More environmental and sequencing studies are needed to further understand the genetic diversity, evolution and virulence of these emerging organisms.
Sections du résumé
BACKGROUND
The soil-dwelling bacillus Burkholderia pseudomallei is the etiological-agent of the neglected and life-threatening emerging infection melioidosis. The distribution of B. pseudomallei in West Africa is unknown. In the present study we aimed to determine whether B. pseudomallei and B. thailandensis are present in the environment of central Sierra Leone.
METHODOLOGY/PRINCIPAL FINDINGS
In June-July 2017, we conducted an environmental surveillance study-designed in accordance with existing consensus guidelines-in central Sierra Leone. A total of 1,000 soil samples (100 per site) were collected and cultured. B. pseudomallei was not identified in the soil, but we identified seven novel B. thailandensis sequence types with multi-locus sequence typing (MLST) and 16S rRNA gene sequence analyses.
CONCLUSIONS/SIGNIFICANCE
The presence of B. pseudomallei was not demonstrated, however, multiple novel B. thailandensis sequence types were identified. More environmental and sequencing studies are needed to further understand the genetic diversity, evolution and virulence of these emerging organisms.
Identifiants
pubmed: 31199807
doi: 10.1371/journal.pntd.0007402
pii: PNTD-D-19-00006
pmc: PMC6623504
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Ribosomal
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0007402Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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