Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2019
Historique:
received: 12 12 2018
accepted: 15 05 2019
entrez: 28 6 2019
pubmed: 28 6 2019
medline: 12 2 2020
Statut: epublish

Résumé

Over the last decades, endophytic fungi represent a new source of pharmacologically active secondary metabolites based on the underlying assumption that they live symbiotically within their plant host. In the present study, a new endophytic fungus was isolated from Rauwolfia macrophylla, a medicinal plant from Cameroon. The fungus showed a highest homology to Curvularia sp. based on complete nucleotide sequence data generated from the internal transcribed spacer (ITS) of ribosomal DNA region. Large scale fermentation, working-up and separation of the strain extract using different chromatographic techniques afforded three bioactive compounds: 2'-deoxyribolactone (1), hexylitaconic acid (2) and ergosterol (3). The chemical structures of compounds 1-3 were confirmed by 1 and 2D NMR spectroscopy and mass spectrometry, and comparison with corresponding literature data. Biologically, the antimicrobial, antioxidant activities and the acetylcholinesterase inhibitory of the isolated compounds were studied.

Identifiants

pubmed: 31247016
doi: 10.1371/journal.pone.0217627
pii: PONE-D-18-35553
pmc: PMC6597039
doi:

Substances chimiques

Antioxidants 0
Cholinesterase Inhibitors 0
Free Radical Scavengers 0
Phytochemicals 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0217627

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Fatma Kaaniche (F)

Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany.
Laboratory of Organic Chemistry, Natural Substances Team, Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia.
Laboratory of Microorganisms and Biomolecules of the Centre of Biotechnology of Sfax-Tunisia, Sfax, Tunisia.

Abdelaaty Hamed (A)

Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany.
Chemistry Department, Faculty of Science, Al-Azhar University, Nasr City-Cairo, Egypt.

Ahmed S Abdel-Razek (AS)

Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany.
Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki-Giza, Egypt.

Daniel Wibberg (D)

Center of Biotechnology(CeBiTec), Bielefeld University, Bielefeld, Germany.

Negera Abdissa (N)

Department of Chemistry, Jimma University, Jimma, Ethiopia.

Imene Zendah El Euch (IZ)

Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany.

Noureddine Allouche (N)

Laboratory of Organic Chemistry, Natural Substances Team, Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia.

Lotfi Mellouli (L)

Laboratory of Microorganisms and Biomolecules of the Centre of Biotechnology of Sfax-Tunisia, Sfax, Tunisia.

Mohamed Shaaban (M)

Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany.
Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki-Cairo, Egypt.

Nobert Sewald (N)

Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany.

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Classifications MeSH