Microbiome dynamics and phaC synthase genes selected in a pilot plant producing polyhydroxyalkanoate from the organic fraction of urban waste.
16S rRNA gene high-throughput sequencing
Mixed microbial culture (MMC)
Organic Fraction of Municipal Solid Waste (OFMSW)
Polyhydroxyalkanoates (PHA)
Sewage sludge (SS)
phaC synthase genes
Journal
The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500
Informations de publication
Date de publication:
01 Nov 2019
01 Nov 2019
Historique:
received:
13
06
2019
revised:
28
06
2019
accepted:
28
06
2019
pubmed:
8
7
2019
medline:
28
11
2019
entrez:
8
7
2019
Statut:
ppublish
Résumé
This study analyses the bacterial population dynamics of a mixed microbial community (MMC) selected in a pilot plant producing polyhydroxyalkanoate (PHA) from the fermentation of the organic fraction of urban waste (OFMSW) and sewage sludge (SS). 16S rRNA gene high-throughput sequencing revealed the occurrence of a variety of PHA accumulating bacteria that ensured a stable PHA production in an open system operating with real substrates and without temperature control. The Volatile Fatty Acids (VFA) changes in the feed and the temperature variation affected the dynamics of the PHA-accumulating bacteria over the plant operation. Remarkably, the higher PHA content was associated to a MMC largely comprising of Hydrogenophaga species during the operation at higher working temperature. The involvement of a heterogeneous PHA-accumulating MMC was associated with a high phaC synthase genes biodiversity confirming the occurrence of a functional redundancy.
Identifiants
pubmed: 31280158
pii: S0048-9697(19)33064-5
doi: 10.1016/j.scitotenv.2019.06.491
pii:
doi:
Substances chimiques
Polyhydroxyalkanoates
0
RNA, Bacterial
0
RNA, Ribosomal, 16S
0
Sewage
0
Waste Water
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
765-773Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.