Isolation, Purification, Characterization and Direct Conjugation of the Lipid A-Free Lipopolysaccharide of Vibrio cholerae O139.
NMR spectroscopy
Vibrio cholerae
glycoconjugate vaccines
polysaccharides
squaric acid
Journal
Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783
Informations de publication
Date de publication:
08 10 2019
08 10 2019
Historique:
received:
31
05
2019
revised:
09
07
2019
pubmed:
16
7
2019
medline:
24
10
2019
entrez:
16
7
2019
Statut:
ppublish
Résumé
The lipopolysaccharide (LPS) of Vibrio cholerae O139, strain CIRS245, was isolated conventionally, and the lipid A was removed by mild acid hydrolysis (0.1 m NaOAc buffer containing 1 % SDS, pH 4.2, 95 °C, 8 h). The crude product was a complex mixture consisting mainly of constituent fragments of the O-specific polysaccharide-core (OSPc). The OSPc was only a minor component in the mixture. Two-stage purification of the crude OSPc by HPLC gave pure OSPc fragment of the LPS, as shown by NMR spectroscopy, analytical HPLC and ESI-MS. This material is the purest OSPc fragment of the LPS from Vibrio cholerae O139 reported to date. The purified OSPc was readily converted to the corresponding methyl squarate derivative and the latter was conjugated to BSA. The conjugate, when examined by ELISA, showed immunoreactivity with sera from patients in Bangladesh recovering from cholera caused by V. cholerae O139, but not O1.
Identifiants
pubmed: 31306528
doi: 10.1002/chem.201902263
pmc: PMC6783332
mid: NIHMS1046333
doi:
Substances chimiques
Lipid A
0
Lipopolysaccharides
0
Sodium Acetate
4550K0SC9B
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12946-12956Subventions
Organisme : Intramural NIH HHS
ID : Z01 DK059701
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI106878
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA DK059701-45
Pays : United States
Organisme : NIAID NIH HHS
ID : R37 AI106878
Pays : United States
Organisme : Intramural NIH HHS
ID : Z99 DK999999
Pays : United States
Organisme : NIDDK NIH HHS
ID : DK059701
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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