Genome-wide in silico identification of LysM-RLK genes in potato (Solanum tuberosum L.).
CERK
LysM-RLK
Plant disease resistance
Potato
Receptors
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
25
02
2019
accepted:
27
06
2019
pubmed:
19
7
2019
medline:
10
3
2020
entrez:
19
7
2019
Statut:
ppublish
Résumé
The receptor like kinases (RLKs) belong to the RLK/Pelle superfamily, one of the largest gene families in plants. RLKs play an important role in plant development, as well as in response to biotic and abiotic stresses. The lysine motif receptor like kinases (LysM-RLKs) are a subfamily of RLKs containing at least one lysine motif (LysM) that are involved in the perception of elicitors or pathogen-associated molecular patterns (PAMPs). In the present study, 77 putative RLKs genes and three receptor like proteins were identified in potato (Solanum tuberosum) genome, following a genome-wide search. The 77 potato RLK proteins are classified into two major phylogenetic groups based on their kinase domain amino acid sequence similarities. Out of 77 RLKs, 10 proteins had at least one LysM. Among them three RLP proteins were found in potato genome with either 2 or three tandem LysM but these lacked a cytoplasmic kinase domain. Expression analyses of a potato LysM-RLKs (StLysM-RLK05) was carried out by a Real time RT-PCR, following inoculation of potato leaves and immature tubers with late blight and common scab pathogens, respectively. The expression was significantly higher in resistant than in susceptible following S. scabies inoculation. The StLysM-RLK05 sequence was verified and it was polymorphic in scab susceptible cultivar. The present study provides an overview of the StLysM-RLKs gene family in potato genome. This information is helpful for future functional analysis of such an important protein family, in Solanaceae species.
Identifiants
pubmed: 31317454
doi: 10.1007/s11033-019-04951-z
pii: 10.1007/s11033-019-04951-z
doi:
Substances chimiques
Plant Proteins
0
Protein Kinases
EC 2.7.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5005-5017Subventions
Organisme : Natural Sciences and Engineering Research Council of Canada
ID : RGPIN/05063-2016
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