A comparison of the nitrogen metabolic networks of Kluyveromyces marxianus and Saccharomyces cerevisiae.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
09
04
2019
revised:
20
06
2019
accepted:
18
07
2019
pubmed:
25
7
2019
medline:
14
5
2020
entrez:
24
7
2019
Statut:
ppublish
Résumé
In grape must, nitrogen is available as a complex mixture of various compounds (ammonium and amino acids). Wine yeasts assimilate these multiple sources in order to suitably fulfil their anabolic requirements during alcoholic fermentation. Nevertheless, the order of uptake and the intracellular fate of these sources are likely to differ between strains and species. Using a two-pronged strategy of isotopic filiation and RNA sequencing, the metabolic network of nitrogen utilization and its regulation in Kluyveromyces marxianus were described, in comparison with those of Saccharomyces cerevisiae. The data highlighted differences in the assimilation of ammonium and arginine between the two species. The data also revealed that the metabolic fate of certain nitrogen sources differed, thereby resulting in the production of various amounts of key wine aroma compounds. These observations were corroborated by the gene expression analysis.
Identifiants
pubmed: 31336027
doi: 10.1111/1462-2920.14756
doi:
Substances chimiques
Amino Acids
0
Ammonium Compounds
0
Nitrogen
N762921K75
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4076-4091Subventions
Organisme : International Committee on Food Microbiology and Hygiene
Pays : International
Organisme : Lallemand Inc.
Pays : International
Organisme : National Research Foundation
ID : 83471
Pays : International
Informations de copyright
© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.
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