The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport.
MLLE domain
PAM2 motif
endosome
microtubule
poly(A)-binding protein
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
09
11
2018
revised:
14
06
2019
accepted:
21
06
2019
pubmed:
25
7
2019
medline:
2
6
2020
entrez:
25
7
2019
Statut:
ppublish
Résumé
mRNA transport determines spatiotemporal protein expression. Transport units are higher-order ribonucleoprotein complexes containing cargo mRNAs, RNA-binding proteins and accessory proteins. Endosomal mRNA transport in fungal hyphae belongs to the best-studied translocation mechanisms. Although several factors are known, additional core components are missing. Here, we describe the 232 kDa protein Upa2 containing multiple PAM2 motifs (poly[A]-binding protein [Pab1]-associated motif 2) as a novel core component. Loss of Upa2 disturbs transport of cargo mRNAs and associated Pab1. Upa2 is present on almost all transport endosomes in an mRNA-dependent manner. Surprisingly, all four PAM2 motifs are dispensable for function during unipolar hyphal growth. Instead, Upa2 harbours a novel N-terminal effector domain as important functional determinant as well as a C-terminal GWW motif for specific endosomal localisation. In essence, Upa2 meets all the criteria of a novel core component of endosomal mRNA transport and appears to carry out crucial scaffolding functions.
Identifiants
pubmed: 31338952
doi: 10.15252/embr.201847381
pmc: PMC6726905
doi:
Substances chimiques
Fungal Proteins
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e47381Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : FE448/5-2 DFG-FOR1334
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : FE448/10-1 DFG-FOR2333
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : DFG-CRC1208 project number 267205415
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : CEPLAS EXC1028
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : FE448/9-1
Pays : International
Informations de copyright
© 2019 The Authors.
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