Differential homotypic and heterotypic interactions of antigen 43 (Ag43) variants in autotransporter-mediated bacterial autoaggregation.
Adhesins, Escherichia coli
/ genetics
Antigenic Variation
/ genetics
Antigens, Bacterial
/ genetics
Bacterial Adhesion
/ genetics
Bacterial Outer Membrane Proteins
/ genetics
Biofilms
/ growth & development
Biological Transport
/ genetics
Escherichia coli
/ genetics
Escherichia coli Proteins
/ genetics
Phylogeny
Type V Secretion Systems
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
31 07 2019
31 07 2019
Historique:
received:
05
03
2019
accepted:
18
06
2019
entrez:
2
8
2019
pubmed:
2
8
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Antigen 43 (Ag43) is a cell-surface exposed protein of Escherichia coli secreted by the Type V, subtype a, secretion system (T5aSS) and belonging to the family of self-associating autotransporters (SAATs). These modular proteins, comprising a cleavable N-terminal signal peptide, a surface-exposed central passenger and an outer membrane C-terminal translocator, self-recognise in a Velcro-like handshake mechanism. A phylogenetic network analysis focusing on the passenger revealed for the first time that they actually distribute into four distinct classes, namely C1, C2, C3 and C4. Structural alignment and modelling analyses demonstrated these classes arose from shuffling of two different subdomains within the Ag43 passengers. Functional analyses revealed that homotypic interactions occur for all Ag43 classes but significant differences in the sedimentation kinetics and aggregation state were present when Ag43
Identifiants
pubmed: 31367003
doi: 10.1038/s41598-019-47608-4
pii: 10.1038/s41598-019-47608-4
pmc: PMC6668479
doi:
Substances chimiques
Adhesins, Escherichia coli
0
Antigens, Bacterial
0
Bacterial Outer Membrane Proteins
0
Escherichia coli Proteins
0
Type V Secretion Systems
0
antigen 43, E coli
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11100Références
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