Molecular epidemiological analysis and risk factors for acquisition of carbapenemase-producing
Adolescent
Adult
Aged
Aged, 80 and over
Bacterial Proteins
/ genetics
Carbapenem-Resistant Enterobacteriaceae
/ classification
Chaperonin 60
/ genetics
Child
Child, Preschool
Cross Infection
/ epidemiology
Enterobacter cloacae
/ genetics
Enterobacteriaceae Infections
/ epidemiology
Female
Humans
Infant
Infant, Newborn
Japan
/ epidemiology
Length of Stay
Male
Middle Aged
Molecular Epidemiology
Multilocus Sequence Typing
Phylogeny
Plasmids
/ genetics
Retrospective Studies
Whole Genome Sequencing
/ methods
Young Adult
beta-Lactamases
/ genetics
Carbapenem-resistant Enterobacteriaceae
Carbapenemase
Carbapenemase-producing Enterobacteriaceae
Enterobacter cloacae complex
Molecular epidemiology
Multilocus sequence typing
Plasmid
Repetitive extragenic palindromic polymerase chain reaction
Whole-genome sequencing
β-Lactamase
Journal
Antimicrobial resistance and infection control
ISSN: 2047-2994
Titre abrégé: Antimicrob Resist Infect Control
Pays: England
ID NLM: 101585411
Informations de publication
Date de publication:
2019
2019
Historique:
received:
17
04
2019
accepted:
15
07
2019
entrez:
2
8
2019
pubmed:
2
8
2019
medline:
26
6
2020
Statut:
epublish
Résumé
To clarify the molecular epidemiology of carbapenem-resistant Using clinical CREC isolates detected in a Japanese university hospital over 4 years, carbapenemase production was screened with phenotypic methods. Carbapenemase genes were analysed by PCR and sequencing. Molecular epidemiological analyses were conducted with repetitive extragenic palindromic (REP)-PCR and multilocus sequence typing (MLST). CRECs were identified to the subspecies level by Thirty-nine CRECs including 20 CPECs carrying A longer hospital stay, presence of a urinary catheter, and intubation are risk factors for CPEC acquisition. In addition to horizontal transmission of genetically indistinguishable CPECs, IncHI2A plasmid carrying
Sections du résumé
Background
To clarify the molecular epidemiology of carbapenem-resistant
Methods
Using clinical CREC isolates detected in a Japanese university hospital over 4 years, carbapenemase production was screened with phenotypic methods. Carbapenemase genes were analysed by PCR and sequencing. Molecular epidemiological analyses were conducted with repetitive extragenic palindromic (REP)-PCR and multilocus sequence typing (MLST). CRECs were identified to the subspecies level by
Results
Thirty-nine CRECs including 20 CPECs carrying
Conclusions
A longer hospital stay, presence of a urinary catheter, and intubation are risk factors for CPEC acquisition. In addition to horizontal transmission of genetically indistinguishable CPECs, IncHI2A plasmid carrying
Identifiants
pubmed: 31367347
doi: 10.1186/s13756-019-0578-3
pii: 578
pmc: PMC6657070
doi:
Substances chimiques
Bacterial Proteins
0
Chaperonin 60
0
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Types de publication
Journal Article
Observational Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
126Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no competing interests.
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