Evolution of l-DOPA 4,5-dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales.
Caryophyllales
anthocyanins
betalains
convergent evolution
gene duplication
l-DOPA 4, 5-dioxygenase (DODA)
metabolic operon
plant pigments
specialised metabolism
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
30
05
2019
accepted:
22
07
2019
pubmed:
2
8
2019
medline:
15
5
2021
entrez:
2
8
2019
Statut:
ppublish
Résumé
The evolution of l-DOPA 4,5-dioxygenase activity, encoded by the gene DODA, was a key step in the origin of betalain biosynthesis in Caryophyllales. We previously proposed that l-DOPA 4,5-dioxygenase activity evolved via a single Caryophyllales-specific neofunctionalisation event within the DODA gene lineage. However, this neofunctionalisation event has not been confirmed and the DODA gene lineage exhibits numerous gene duplication events, whose evolutionary significance is unclear. To address this, we functionally characterised 23 distinct DODA proteins for l-DOPA 4,5-dioxygenase activity, from four betalain-pigmented and five anthocyanin-pigmented species, representing key evolutionary transitions across Caryophyllales. By mapping these functional data to an updated DODA phylogeny, we then explored the evolution of l-DOPA 4,5-dioxygenase activity. We find that low l-DOPA 4,5-dioxygenase activity is distributed across the DODA gene lineage. In this context, repeated gene duplication events within the DODA gene lineage give rise to polyphyletic occurrences of elevated l-DOPA 4,5-dioxygenase activity, accompanied by convergent shifts in key functional residues and distinct genomic patterns of micro-synteny. In the context of an updated organismal phylogeny and newly inferred pigment reconstructions, we argue that repeated convergent acquisition of elevated l-DOPA 4,5-dioxygenase activity is consistent with recurrent specialisation to betalain synthesis in Caryophyllales.
Identifiants
pubmed: 31369159
doi: 10.1111/nph.16089
pmc: PMC7384185
doi:
Substances chimiques
Betalains
37279-84-8
Levodopa
46627O600J
Dioxygenases
EC 1.13.11.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
914-929Subventions
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
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