The population structure of Clostridium tetani deduced from its pan-genome.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
02 08 2019
02 08 2019
Historique:
received:
26
12
2018
accepted:
16
07
2019
entrez:
4
8
2019
pubmed:
4
8
2019
medline:
20
11
2020
Statut:
epublish
Résumé
Clostridium tetani produces a potent neurotoxin, the tetanus neurotoxin (TeNT) that is responsible for the worldwide neurological disease tetanus, but which can be efficiently prevented by vaccination with tetanus toxoid. Until now only one type of TeNT has been characterized and very little information exists about the heterogeneity among C. tetani strains. We report here the genome sequences of 26 C. tetani strains, isolated between 1949 and 2017 and obtained from different locations. Genome analyses revealed that the C. tetani population is distributed in two phylogenetic clades, a major and a minor one, with no evidence for clade separation based on geographical origin or time of isolation. The chromosome of C. tetani is highly conserved; in contrast, the TeNT-encoding plasmid shows substantial heterogeneity. TeNT itself is highly conserved among all strains; the most relevant difference is an insertion of four amino acids in the C-terminal receptor-binding domain in four strains that might impact on receptor-binding properties. Other putative virulence factors, including tetanolysin and collagenase, are encoded in all genomes. This study highlights the population structure of C. tetani and suggests that tetanus-causing strains did not undergo extensive evolutionary diversification, as judged from the high conservation of its main virulence factors.
Identifiants
pubmed: 31375706
doi: 10.1038/s41598-019-47551-4
pii: 10.1038/s41598-019-47551-4
pmc: PMC6677821
doi:
Substances chimiques
Neurotoxins
0
Tetanus Toxin
0
Virulence Factors
0
tetanolysin
61614-31-1
Collagenases
EC 3.4.24.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11220Commentaires et corrections
Type : ErratumIn
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