Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.


Journal

Oncogene
ISSN: 1476-5594
Titre abrégé: Oncogene
Pays: England
ID NLM: 8711562

Informations de publication

Date de publication:
10 2020
Historique:
received: 04 03 2019
accepted: 05 04 2019
revised: 04 04 2019
pubmed: 7 8 2019
medline: 15 12 2020
entrez: 7 8 2019
Statut: ppublish

Résumé

Advanced Bladder Cancer (BLCA) remains a clinical challenge that lacks effective therapeutic measures. Here, we show that distinct, stage-wise metabolic alterations in BLCA are associated with the loss of function of aldehyde oxidase (AOX1). AOX1 associated metabolites have a high predictive value for advanced BLCA and our findings demonstrate that AOX1 is epigenetically silenced during BLCA progression by the methyltransferase activity of EZH2. Knockdown (KD) of AOX1 in normal bladder epithelial cells re-wires the tryptophan-kynurenine pathway resulting in elevated NADP levels which may increase metabolic flux through the pentose phosphate (PPP) pathway, enabling increased nucleotide synthesis, and promoting cell invasion. Inhibition of NADP synthesis rescues the metabolic effects of AOX1 KD. Ectopic AOX1 expression decreases NADP production, PPP flux and nucleotide synthesis, while decreasing invasion in cell line models and suppressing growth in tumor xenografts. Further gain and loss of AOX1 confirm the EZH2-dependent activation, metabolic deregulation, and tumor growth in BLCA. Our findings highlight the therapeutic potential of AOX1 and provide a basis for the development of prognostic markers for advanced BLCA.

Identifiants

pubmed: 31383940
doi: 10.1038/s41388-019-0902-7
pii: 10.1038/s41388-019-0902-7
pmc: PMC8058741
mid: NIHMS1660300
doi:

Substances chimiques

Nucleotides 0
Kynurenine 343-65-7
NADP 53-59-8
Tryptophan 8DUH1N11BX
AOX1 protein, human EC 1.2.3.1
Aldehyde Oxidase EC 1.2.3.1
EZH2 protein, human EC 2.1.1.43
Enhancer of Zeste Homolog 2 Protein EC 2.1.1.43

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6265-6285

Subventions

Organisme : NCI NIH HHS
ID : R01 CA133458
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA125123
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA142543
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK056338
Pays : United States
Organisme : NIH HHS
ID : S10 OD020151
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA216426
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA167234
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA220297
Pays : United States

Commentaires et corrections

Type : CommentIn
Type : ErratumIn

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Auteurs

Venkatrao Vantaku (V)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.

Vasanta Putluri (V)

Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.

David A Bader (DA)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.

Suman Maity (S)

Department of Biostatistics, School of Public Health, University of Texas, Houston, TX, USA.

Jing Ma (J)

Fred Hutchinson Cancer Research Center, Division of Public Health Sciences, Seattle, WA, USA.

James M Arnold (JM)

Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA.

Kimal Rajapakshe (K)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.

Sri Ramya Donepudi (SR)

Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.

Friedrich-Carl von Rundstedt (FC)

Scott Department of Urology, Baylor College of Medicine, Houston, TX, USA.
Department of Urology, Jena University Hospital, Friedrich-Schiller-University, Jena, Germany.

Vaishnavi Devarakonda (V)

Lester and Sue Smith Breast Center, Baylor College of Medicine, Houston, TX, USA.

Julien Dubrulle (J)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.
Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.

Balasubramanyam Karanam (B)

Department of Biology and Center for Cancer Research, Tuskegee University, Tuskegee, AL, USA.

Sean E McGuire (SE)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.

Fabio Stossi (F)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.
Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.

Abhinav K Jain (AK)

Center for Cancer Epigenetics, Department of Epigenetics and Molecular Carcinogenesis, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.

Cristian Coarfa (C)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.
Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.

Qi Cao (Q)

Center for Inflammation and Epigenetics, Institute for Academic Medicine Houston Methodist Research Institute, Houston, TX, USA.

Andrew G Sikora (AG)

Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.
Department of Otolaryngology-Head & Neck Surgery, Baylor College of Medicine, Houston, TX, USA.

Hugo Villanueva (H)

Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.
Department of Otolaryngology-Head & Neck Surgery, Baylor College of Medicine, Houston, TX, USA.

Shyam M Kavuri (SM)

Lester and Sue Smith Breast Center, Baylor College of Medicine, Houston, TX, USA.

Yair Lotan (Y)

Department of Urology, University of Texas Southwestern, Dallas, TX, USA.

Arun Sreekumar (A)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.
Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA.
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA.

Nagireddy Putluri (N)

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA. putluri@bcm.edu.
Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, TX, USA. putluri@bcm.edu.

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