Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli.
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
19 Aug 2019
19 Aug 2019
Historique:
received:
19
06
2019
accepted:
03
08
2019
entrez:
21
8
2019
pubmed:
21
8
2019
medline:
31
12
2019
Statut:
epublish
Résumé
Acrylic acid (AA) is a widely used commodity chemical derived from non-renewable fossil fuel sources. Alternative microbial-based production methodologies are being developed with the aim of providing "green" acrylic acid. These initiatives will benefit from component sensing tools that facilitate rapid and easy detection of in vivo AA production. We developed a novel transcriptional sensor facilitating in vivo detection of acrylic acid (AA). RNAseq analysis of Escherichia coli exposed to sub-lethal doses of acrylic acid identified a selectively responsive promoter (P The transcriptional AA sensor developed in this study will benefit strain, enzyme and pathway engineering initiatives targeting the efficient formation of bio-acrylic acid.
Sections du résumé
BACKGROUND
BACKGROUND
Acrylic acid (AA) is a widely used commodity chemical derived from non-renewable fossil fuel sources. Alternative microbial-based production methodologies are being developed with the aim of providing "green" acrylic acid. These initiatives will benefit from component sensing tools that facilitate rapid and easy detection of in vivo AA production.
RESULTS
RESULTS
We developed a novel transcriptional sensor facilitating in vivo detection of acrylic acid (AA). RNAseq analysis of Escherichia coli exposed to sub-lethal doses of acrylic acid identified a selectively responsive promoter (P
CONCLUSIONS
CONCLUSIONS
The transcriptional AA sensor developed in this study will benefit strain, enzyme and pathway engineering initiatives targeting the efficient formation of bio-acrylic acid.
Identifiants
pubmed: 31426802
doi: 10.1186/s12934-019-1185-y
pii: 10.1186/s12934-019-1185-y
pmc: PMC6699081
doi:
Substances chimiques
Acrylates
0
Acrylamide
20R035KLCI
acrylic acid
J94PBK7X8S
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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