Development and characterization of 49 novel microsatellite markers in the African catfish, Clarias gariepinus (Burchell, 1822).
African catfish
Anthropogenic effect
Clarias gariepinus
Genetic diversity
Microsatellite
Population genetics
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
16
01
2019
accepted:
04
09
2019
pubmed:
20
9
2019
medline:
24
4
2020
entrez:
20
9
2019
Statut:
ppublish
Résumé
The African catfish or sharp tooth catfish (Clarias gariepinus) is one of the important species (due to its high environmental tolerance and easily controllable breeding habits) that can significantly contribute to reducing hunger in many countries. It is farmed in numerous African, Asian, and European countries. Moreover, during the last decades its production has grown significantly worldwide. Currently, following the carp, this species is produced in the second largest volume in Hungary. Despite its economic importance, the stocks have been maintained without genetic control or guided breeding. Molecular genetic data on bred populations or strains are very limited. In order to investigate the genetic structure of the stocks, 49 new microsatellite markers were characterized and tested on 32 individuals from a Hungarian farmed stock. All these markers were polymorph. The number of alleles per locus ranged from 2 to 11. The observed and expected overall heterozygosities were between 0.519 and 0.544 respectively and the overall inbreeding coefficient (Fis: 0.063) does not reveal the presence of inbreeding. However, 63% of the markers showed significant deviations from HWE. The results suggest that the maintenance of genetic variation within the stock require high attention in closed bred populations. These new markers provide a useful tool for population and conservation genetics of natural and bred African catfish populations.
Identifiants
pubmed: 31535323
doi: 10.1007/s11033-019-05062-5
pii: 10.1007/s11033-019-05062-5
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6599-6608Subventions
Organisme : Országos Tudományos Kutatási Alapprogramok (HU)
ID : K105393
Organisme : Emberi Eroforrások Minisztériuma
ID : FEKUT2019: TUDFO/47138/2019-ITM
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