Protein engineering expands the effector recognition profile of a rice NLR immune receptor.
E. coli
NLR receptor
molecular biophysics
plant biology
plant disease resistance
protein engineering
protein structure
rice
rice blast
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
19 09 2019
19 09 2019
Historique:
received:
17
04
2019
accepted:
17
09
2019
pubmed:
20
9
2019
medline:
20
2
2020
entrez:
20
9
2019
Statut:
epublish
Résumé
Plant nucleotide binding, leucine-rich repeat (NLR) receptors detect pathogen effectors and initiate an immune response. Since their discovery, NLRs have been the focus of protein engineering to improve disease resistance. However, this approach has proven challenging, in part due to their narrow response specificity. Previously, we revealed the structural basis of pathogen recognition by the integrated heavy metal associated (HMA) domain of the rice NLR Pikp (Maqbool et al., 2015). Here, we used structure-guided engineering to expand the response profile of Pikp to variants of the rice blast pathogen effector AVR-Pik. A mutation located within an effector-binding interface of the integrated Pikp-HMA domain increased the binding affinity for AVR-Pik variants in vitro and in vivo. This translates to an expanded cell-death response to AVR-Pik variants previously unrecognized by Pikp in planta. The structures of the engineered Pikp-HMA in complex with AVR-Pik variants revealed the mechanism of expanded recognition. These results provide a proof-of-concept that protein engineering can improve the utility of plant NLR receptors where direct interaction between effectors and NLRs is established, particularly where this interaction occurs via integrated domains.
Identifiants
pubmed: 31535976
doi: 10.7554/eLife.47713
pii: 47713
pmc: PMC6768660
doi:
pii:
Substances chimiques
Antigens, Bacterial
0
NLR Proteins
0
Plant Proteins
0
Receptors, Immunologic
0
Recombinant Proteins
0
Banques de données
PDB
['6R8K', '6R8M']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/J004553
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P012574
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M02198X
Pays : United Kingdom
Organisme : H2020 European Research Council
ID : 743165
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 15H05779
Pays : International
Informations de copyright
© 2019, De la Concepcion et al.
Déclaration de conflit d'intérêts
JD, MF, DM, RT, SK, MB No competing interests declared
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