Short communication: An inducible CRISPR/dCas9 gene repression system in Lactococcus lactis.

CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats / genetics Gene Targeting Lactobacillales / enzymology Lactococcus lactis / enzymology Nisin / metabolism Plasmids / genetics Promoter Regions, Genetic / genetics RNA, Guide, Kinetoplastida / genetics
CRISPR interference Lactococcus lactis gene repression inducible dCas9

Journal

Journal of dairy science
ISSN: 1525-3198
Titre abrégé: J Dairy Sci
Pays: United States
ID NLM: 2985126R

Informations de publication

Date de publication:
Jan 2020
Historique:
received: 28 07 2019
accepted: 30 08 2019
pubmed: 18 11 2019
medline: 9 4 2020
entrez: 18 11 2019
Statut: ppublish

Résumé

Lactococcus lactis, one of the most important probiotic lactic acid bacteria (LAB), is widely used in the dairy industry as a cell factory for recombinant protein production. Currently, a nisin-controlled inducible expression system is used for this purpose and represents the only commercial expression system in LAB. However, the available genetic modification methods are rather limited for modulating gene expression in L. lactis. Here, we developed a 2-plasmid system for gene transcription repression in L. lactis NZ9000 that uses inducible clustered regularly interspaced short palindromic repeats (CRISPR)-dCas9. An inducible promoter P

Identifiants

DOI: 10.3168/jds.2019-17346 PMID: 31733872
pubmed: 31733872
pii: S0022-0302(19)31011-2
doi: 10.3168/jds.2019-17346
pii:
doi:

Substances chimiques

RNA, Guide 0
Nisin 1414-45-5
nisin A EN8XKG133D

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

161-165

Informations de copyright

Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Auteurs

Zhi-Qiang Xiong (ZQ)

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Yun-Ying Wei (YY)

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Ling-Hui Kong (LH)

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Xin Song (X)

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Hua-Xi Yi (HX)

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

Lian-Zhong Ai (LZ)

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China. Electronic address: ailianzhong@hotmail.com.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Short communication: An inducible CRISPR/dCas9...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › Short communication: An inducible CRISPR/dCas9...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Short communication: An inducible CRISPR/dCas9...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Short communication: An inducible CRISPR/dCas9...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Short communication: An inducible CRISPR/dCas9...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Perforines › Bactériocines › Nisine › Short communication: An inducible CRISPR/dCas9...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Short communication: An inducible CRISPR/dCas9...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › Short communication: An inducible CRISPR/dCas9...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › ARN guide › Short communication: An inducible CRISPR/dCas9...