Metatranscriptomic reconstruction reveals RNA viruses with the potential to shape carbon cycling in soil.
metatranscriptome
phage
rhizosphere
soil
virus
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
17 12 2019
17 12 2019
Historique:
pubmed:
28
11
2019
medline:
2
4
2020
entrez:
28
11
2019
Statut:
ppublish
Résumé
Viruses impact nearly all organisms on Earth, with ripples of influence in agriculture, health, and biogeochemical processes. However, very little is known about RNA viruses in an environmental context, and even less is known about their diversity and ecology in soil, 1 of the most complex microbial systems. Here, we assembled 48 individual metatranscriptomes from 4 habitats within a planted soil sampled over a 22-d time series: Rhizosphere alone, detritosphere alone, rhizosphere with added root detritus, and unamended soil (4 time points and 3 biological replicates). We resolved the RNA viral community, uncovering a high diversity of viral sequences. We also investigated possible host organisms by analyzing metatranscriptome marker genes. Based on viral phylogeny, much of the diversity was
Identifiants
pubmed: 31772013
pii: 1908291116
doi: 10.1073/pnas.1908291116
pmc: PMC6926006
doi:
Substances chimiques
Soil
0
Carbon
7440-44-0
Banques de données
GENBANK
['MN032676', 'MN036333', 'MK945893', 'MK946421']
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
25900-25908Informations de copyright
Copyright © 2019 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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