Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii.
Algal Proteins
/ genetics
Amino Acid Sequence
Chlamydomonas reinhardtii
/ chemistry
Glycoproteins
/ chemistry
Glycosylation
Mass Spectrometry
Mutagenesis, Insertional
Pentosyltransferases
/ genetics
Phylogeny
Polysaccharides
/ chemistry
Sequence Alignment
Xylose
/ chemistry
UDP Xylose-Protein Xylosyltransferase
Chlamydomonas reinhardtii
N-glycosylation
insertional mutants
ion mobility
mass spectrometry
xylosylation
xylosyltransferases
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
20
05
2019
revised:
24
10
2019
accepted:
05
11
2019
pubmed:
30
11
2019
medline:
18
2
2021
entrez:
29
11
2019
Statut:
ppublish
Résumé
Nowadays, little information is available regarding the N-glycosylation pathway in the green microalga Chlamydomonas reinhardtii. Recent investigation demonstrated that C. reinhardtii synthesizes linear oligomannosides. Maturation of these oligomannosides results in N-glycans that are partially methylated and carry one or two xylose residues. One xylose residue was demonstrated to be a core β(1,2)-xylose. Recently, N-glycoproteomic analysis performed on glycoproteins secreted by C. reinhardtii demonstrated that the xylosyltransferase A (XTA) was responsible for the addition of the core β(1,2)-xylose. Furthermore, another xylosyltransferase candidate named XTB was suggested to be involved in the xylosylation in C. reinhardtii. In the present study, we focus especially on the characterization of the structures of the xylosylated N-glycans from C. reinhardtii taking advantage of insertional mutants of XTA and XTB, and of the XTA/XTB double-mutant. The combination of mass spectrometry approaches allowed us to identify the major N-glycan structures bearing one or two xylose residues. They confirm that XTA is responsible for the addition of the core β(1,2)-xylose, whereas XTB is involved in the addition of the xylose residue onto the linear branch of the N-glycan as well as in the partial addition of the core β(1,2)-xylose suggesting that this transferase exhibits a low substrate specificity. Analysis of the double-mutant suggests that an additional xylosyltransferase is involved in the xylosylation process in C. reinhardtii. Additional putative candidates have been identified in the C. reinhardtii genome. Altogether, these results pave the way for a better understanding of the C. reinhardtii N-glycosylation pathway.
Substances chimiques
Algal Proteins
0
Glycoproteins
0
Polysaccharides
0
Xylose
A1TA934AKO
Pentosyltransferases
EC 2.4.2.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
230-245Informations de copyright
© 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd.
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