Directed evolution by UV-C treatment of Bacillus cereus spores.

Bacillus cereus / genetics Disinfectants Food Microbiology Spores, Bacterial / genetics Ultraviolet Rays
Endospore recalcitrance Germination defect Heat resistance Stress cycling Time-lapse microscopy UV resistance

Journal

International journal of food microbiology
ISSN: 1879-3460
Titre abrégé: Int J Food Microbiol
Pays: Netherlands
ID NLM: 8412849

Informations de publication

Date de publication:
16 Mar 2020
Historique:
received: 13 05 2019
revised: 02 09 2019
accepted: 03 11 2019
pubmed: 4 12 2019
medline: 11 3 2020
entrez: 3 12 2019
Statut: ppublish

Résumé

Bacterial endospores are exposed to a broad variety of sublethal and lethal stresses in the food production chain. Generally, these stresses will not completely eliminate the existing spore populations, and thus constitute a selection pressure on the spores. One stress that is frequently used in the food production chains to disinfect (food) contact surfaces is UV-C. At a wavelength of 254 nm, UV-C has germicidal properties. The aim of this research is to investigate the impact of UV-C stress on the evolution of endospore recalcitrance and germination in B. cereus. A directed evolution experiment was set up in which B. cereus was repeatedly subjected to a cycle of sporulation, sporicidal UV-C treatment, germination and outgrowth. We show here that three independent lineages of UV-C cycled B. cereus spores reproducibly acquired a 30-fold or higher increase in UV-C resistance at 164 mJ/cm

Identifiants

DOI: 10.1016/j.ijfoodmicro.2019.108424 PMID: 31790956
pubmed: 31790956
pii: S0168-1605(19)30355-1
doi: 10.1016/j.ijfoodmicro.2019.108424
pii:
doi:

Substances chimiques

Disinfectants 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

108424

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Katrien Begyn (K)

Research Unit Food Microbiology and Food Preservation (FMFP-UGent), Department of Food Technology, Safety and Health, Part of Food2Know, Faculty Bioscience Engineering, Ghent University, Ghent, Belgium.

Tom Dongmin Kim (TD)

Laboratory of Food Microbiology, Department of Microbial and Molecular systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Leuven, Belgium.

Marc Heyndrickx (M)

ILVO - Flanders Research Institute for Agriculture, Fisheries and Food, Technology and Food Science, Unit - Food Safety, Melle, Belgium; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

Chris Michiels (C)

Laboratory of Food Microbiology, Department of Microbial and Molecular systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Leuven, Belgium; Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Leuven, Belgium.

Abram Aertsen (A)

Laboratory of Food Microbiology, Department of Microbial and Molecular systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Leuven, Belgium. Electronic address: abram.aertsen@kuleuven.be.

Andreja Rajkovic (A)

Research Unit Food Microbiology and Food Preservation (FMFP-UGent), Department of Food Technology, Safety and Health, Part of Food2Know, Faculty Bioscience Engineering, Ghent University, Ghent, Belgium.

Frank Devlieghere (F)

Research Unit Food Microbiology and Food Preservation (FMFP-UGent), Department of Food Technology, Safety and Health, Part of Food2Know, Faculty Bioscience Engineering, Ghent University, Ghent, Belgium. Electronic address: frank.devlieghere@ugent.be.

Articles similaires

UV-C disinfection of ultrasound probes: Challenges of uneven irradiance on complex surfaces.

LaReine Yeoh, Luke Cogar, Mayes Barak et al.
1.00
Ultraviolet Rays Disinfection Ultrasonography Surface Properties Humans

Effects and safety of combined corneal collagen crosslinking and intrastromal corneal ring segment treatment in patients with keratoconus: a retrospective study.

Ali Dal, Mehmet Canleblebici, Bunyamin Kutluksaman et al.
1.00
Humans Keratoconus Male Cross-Linking Reagents Female

Human adipose and umbilical cord mesenchymal stem cell-derived extracellular vesicles mitigate photoaging via TIMP1/Notch1.

Huan Zhang, Xian Xiao, Liping Wang et al.
1.00
Humans Mesenchymal Stem Cells Extracellular Vesicles Skin Aging Tissue Inhibitor of Metalloproteinase-1

Molecular mechanism of flagellar motor rotation arrest in bacterial zoospores of Actinoplanes missouriensis before germination.

Hiromu Kato, Hiroki Tanemura, Tomohiro Kimura et al.
1.00
Flagella Bacterial Proteins Chemotaxis Spores, Bacterial

Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus cereus › Directed evolution by UV-C treatment of...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Désinfectants › Directed evolution by UV-C treatment of...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Qualité alimentaire › Sécurité des aliments › Contamination des aliments › Microbiologie alimentaire › Directed evolution by UV-C treatment of...
questionsmedicales.fr › Stades d'organismes › Spores › Spores bactériens › Directed evolution by UV-C treatment of...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Lumière du soleil › Rayons ultraviolets › Directed evolution by UV-C treatment of...