Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. 'Moscatel Galego').
Alleles
Anthocyanins
/ genetics
Flavonoids
/ genetics
Fruit
/ chemistry
Gene Expression Regulation, Plant
Pigmentation
/ genetics
Plant Proteins
/ genetics
Promoter Regions, Genetic
Retroelements
/ genetics
Secondary Metabolism
/ genetics
Stilbenes
/ metabolism
Transcription Factors
/ genetics
Transcriptome
Vitis
/ chemistry
Berry color
Grapevine
Moscatel Galego
RNA-Seq
Somatic variation
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
09 Dec 2019
09 Dec 2019
Historique:
received:
12
08
2018
accepted:
29
10
2019
entrez:
10
12
2019
pubmed:
10
12
2019
medline:
6
5
2020
Statut:
epublish
Résumé
Somatic mutations occurring within meristems of vegetative propagation material have had a major role in increasing the genetic diversity of the domesticated grapevine (Vitis vinifera subsp. vinifera). The most well studied somatic variation in this species is the one affecting fruit pigmentation, leading to a plethora of different berry skin colors. Color depletion and reversion are often observed in the field. In this study we analyzed the origin of a novel white-to-red skin color reversion and studied its possible metabolic and transcriptomic consequences on cv. 'Muscat à Petits Grains Blancs' (synonym cv. 'Moscatel Galego Branco'), a member of the large family of Muscats. The mild red-skinned variant (cv. 'Muscat à Petits Grains Rouge', synonym cv. 'Moscatel Galego Roxo'), characterized by a preferential accumulation of di-hydroxylated anthocyanins, showed in heterozygosis a partially-excised Gret1 retrotransposon in the promoter region of the MYBA1 anthocyanin regulator, while MYBA2 was still in homozygosis for its non-functional allele. Through metabolic (anthocyanin, resveratrol and piceid quantifications) and transcriptomic (RNA-Seq) analyses, we show that within a near-isogenic background, the transcriptomic consequences of color reversion are largely associated to diminished light/UV-B responses probably as a consequence of the augment of metabolic sunscreens (i.e. anthocyanins). We propose that the reduced activity of the flavonoid tri-hydroxylated sub-branch and decreased anthocyanin synthesis and modification (e.g. methylation and acylation) are the potential causes for the mild red-skinned coloration in the pigmented revertant. The observed positive relation between anthocyanins and stilbenes could be attributable to an increased influx of phenylpropanoid intermediaries due to the replenished activity of MYBA1, an effect yet to be demonstrated in other somatic variants.
Sections du résumé
BACKGROUND
BACKGROUND
Somatic mutations occurring within meristems of vegetative propagation material have had a major role in increasing the genetic diversity of the domesticated grapevine (Vitis vinifera subsp. vinifera). The most well studied somatic variation in this species is the one affecting fruit pigmentation, leading to a plethora of different berry skin colors. Color depletion and reversion are often observed in the field. In this study we analyzed the origin of a novel white-to-red skin color reversion and studied its possible metabolic and transcriptomic consequences on cv. 'Muscat à Petits Grains Blancs' (synonym cv. 'Moscatel Galego Branco'), a member of the large family of Muscats.
RESULTS
RESULTS
The mild red-skinned variant (cv. 'Muscat à Petits Grains Rouge', synonym cv. 'Moscatel Galego Roxo'), characterized by a preferential accumulation of di-hydroxylated anthocyanins, showed in heterozygosis a partially-excised Gret1 retrotransposon in the promoter region of the MYBA1 anthocyanin regulator, while MYBA2 was still in homozygosis for its non-functional allele. Through metabolic (anthocyanin, resveratrol and piceid quantifications) and transcriptomic (RNA-Seq) analyses, we show that within a near-isogenic background, the transcriptomic consequences of color reversion are largely associated to diminished light/UV-B responses probably as a consequence of the augment of metabolic sunscreens (i.e. anthocyanins).
CONCLUSIONS
CONCLUSIONS
We propose that the reduced activity of the flavonoid tri-hydroxylated sub-branch and decreased anthocyanin synthesis and modification (e.g. methylation and acylation) are the potential causes for the mild red-skinned coloration in the pigmented revertant. The observed positive relation between anthocyanins and stilbenes could be attributable to an increased influx of phenylpropanoid intermediaries due to the replenished activity of MYBA1, an effect yet to be demonstrated in other somatic variants.
Identifiants
pubmed: 31815637
doi: 10.1186/s12864-019-6237-5
pii: 10.1186/s12864-019-6237-5
pmc: PMC6902604
doi:
Substances chimiques
Anthocyanins
0
Flavonoids
0
Plant Proteins
0
Retroelements
0
Stilbenes
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
952Subventions
Organisme : Fundação para a Ciência e a Tecnologia
ID : UID/AGR/04033/2019
Organisme : Fundação para a Ciência e Tecnologia
ID : SFRH/BD/96400/2013
Organisme : Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
ID : RTA2014-00016-C02-01
Organisme : Ministerio de Ciencia, Innovación y Universidades
ID : RYC-2017-23645
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