Transcriptomic signature of gut microbiome-contacting cells in colon of spontaneously hypertensive rats.
Adaptor Proteins, Signal Transducing
/ metabolism
Alkaline Phosphatase
/ metabolism
Animals
Blood Pressure
CD3 Complex
/ metabolism
Colon
/ metabolism
Cytokines
/ metabolism
Dysbiosis
Gastrointestinal Microbiome
/ genetics
Hypertension
/ metabolism
Intestinal Mucosa
/ metabolism
Isoenzymes
/ metabolism
Male
Phosphoproteins
/ metabolism
RNA-Seq
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Receptors, Antigen, T-Cell
/ metabolism
Transcriptome
TCR signaling pathway
colonic epithelium
gene expression profile
hypertension
Journal
Physiological genomics
ISSN: 1531-2267
Titre abrégé: Physiol Genomics
Pays: United States
ID NLM: 9815683
Informations de publication
Date de publication:
01 03 2020
01 03 2020
Historique:
pubmed:
24
12
2019
medline:
12
1
2021
entrez:
24
12
2019
Statut:
ppublish
Résumé
Fecal matter transfer from hypertensive patients and animals into normotensive animals increases blood pressure, strengthening the evidence for gut-microbiota interactions in the control of blood pressure. However, cellular and molecular events involved in gut dysbiosis-associated hypertension remain poorly understood. Therefore, our objective in this study was to use gene expression profiling to characterize the gut epithelium layer in the colon in hypertension. We observed significant suppression of components of T cell receptor (TCR) signaling in the colonic epithelium of spontaneously hypertensive rats (SHR) when compared with Wistar Kyoto (WKY) normotensive rats. Western blot analysis confirmed lower expression of key proteins including T cell surface glycoprotein CD3 gamma chain (Cd3g) and lymphocyte cytosolic protein 2 (Lcp2). Furthermore, lower expression of cytokines and receptors responsible for lymphocyte proliferation, differentiation, and activation (e.g.,
Identifiants
pubmed: 31869283
doi: 10.1152/physiolgenomics.00087.2019
pmc: PMC7099409
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
CD3 Complex
0
CD3 antigen, gamma chain
0
Cytokines
0
Isoenzymes
0
Phosphoproteins
0
Receptors, Antigen, T-Cell
0
SLP-76 signal Transducing adaptor proteins
0
Alpi protein, rat
EC 3.1.3.-
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
121-132Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL132448
Pays : United States
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