Optimal fluorescent-dye staining time for the real-time detection of microbes: a study of Saccharomyces cerevisiae.
Auramine-O
DAPI
fluorescent staining
time
yeast
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
09
09
2019
revised:
27
12
2019
accepted:
06
01
2020
pubmed:
12
1
2020
medline:
22
8
2020
entrez:
12
1
2020
Statut:
ppublish
Résumé
To provide information on the time-dependent behaviour of microbe staining by fluorescent dyes in the order of seconds, which is important in terms of the recent rapid and online techniques for microbe measurements and/or environmental microbe analysis. For combinations of yeast (Saccharomyces cerevisiae) and typical dyes, including DAPI (4',6-diamidino-2-phenylindole) and Auramine-O, a suspension of yeast cells in ultrapure water was injected into a dye solution in a micro cuvette placed inside a spectrofluorometer and the fluorescence intensity of the resulting solution was measured at 1 s intervals, starting immediately after the mixing and continued until the time for the maximum intensity using various concentrations of yeast and dyes. The relaxation time τ, which corresponds to ~63·2% of the maximum fluorescence intensity, was shown to decrease to below 1 s with increasing DAPI concentration, whereas it remained constant for 2-3 s with increasing Auramine-O concentration, for example at a yeast concentration of 100 µg ml For the conditions of yeast >10 µg ml Design and operating conditions for rapid and online measurements of microbes can be optimized.
Substances chimiques
Fluorescent Dyes
0
Indoles
0
Benzophenoneidum
2465-27-2
DAPI
47165-04-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1694-1702Subventions
Organisme : Japan Society for the Promotion of Science
ID : 16H02934
Informations de copyright
© 2020 The Society for Applied Microbiology.
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